In this issue...
1997 Santa Fe Highlights
Patrinos Address
Joint Genome Institute (JGI) Comes of Age
JGI Sequencing
JGI Informatics
JGI and Bermuda Quality Sequence
Grants Awarded for JGI Collaboration
JGI Sequencing Clones
Sequencing Strategies,Tools
Gene-Discovery Resources
Sequencing at NIH NHGRI
Functional Genomics
Data Surge Challenges Informaticists
Genome Annotation: Informatics Advances Needed for Age of Functional Genomics
ELSI: Rapid Progress Accelerates Societal Impact of Genome Research
1999 DOE HGP Meeting Set for California
Human Genome Project Administration
New 5-Year Goals, Project Midpoint
DOE, NIH Discuss Informatics
JASON Group Review
BER Genome Instrumentation Research
In the News
Private-Sector Sequencing Plan
Bang for the Buck: Government-Backed Research Underpins Potentially High Payoff Ventures
Palmisano Joins DOE OBER
DNA Files series to be on NPR
HUGO Addresses Sample Collection
Sickle Cell Mice May Lead to New Treatments
TIGR Sequencing 6 More Microbes
Tuberculosis Microbe Sequenced
C. Elegans Sequencing Nears Finish
HGMIS Website Restructured
cDNA Cloning Workshop Identifies Critical Issues
Survey Identifies Growing Need for Synchrotron Analyses
NCGR Announcements
Publications
Report on Functional Consequences of Gene Expression
Book on Tuskegee Conference
Book Focuses on Biomarker Implications, Conference Proceedings
Genome Analysis Protocol Handbook
Software and the Internet
Mouse Genome Informatics Release 2.0
New System Identifies Polymorphisms
DOE Supports Web Site for 1997 AAAS Genome Symposium
Expressed Human Genome Database
Funding
DOE ELSI
NIH NHGRI
NHGRI Initiates Mailing List
U.S. Genome Research Funding
Meeting Calendars & Acronyms
Genome and Biotechnology Meetings
Training Courses and Workshops
Acronyms
HGN archives and subscriptions
HGP Information home
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DOE Refocuses Instrumentation Program
In April the DOE Office of Biological and Environmental Research (OBER) announced its interest in receiving new applications in genome instrumentation research for both substantial evolutionary improvements in current systems and revolutionary technologies for the post-2005 era. To stimulate contributions from investigators not previously involved in DOE's Human Genome Program, OBER invited applications from a broad range of scientists with backgrounds in biology, chemistry, physics, and engineering. At press time, preapplication response had been excellent.
DOE's transition to production sequencing has been based largely on gel electrophoresis, with data acquisition by laser-induced fluorescence. However, this in no way decreases the necessity for innovative long-term basic research in the area of instrumentation support for genome studies. In this context, OBER is refocusing its current Genome Instrumentation Program, taking stock of current progress and considering likely future needs. [See related article on Jason Review.]
To complete the human genome sequence within the available budget and time, substantial improvements in existing sequencing methods would be advantageous. Further, OBER places a strong emphasis on research directed to completely new approaches to genomic analysis. After 2005 the ongoing need will be for fast and cost-effective determination of DNA sequence to compare sequences among individual humans and also to determine the genomes of numerous organisms of biomedical and commercial interest. Additionally, with the continuing acquisition of this remarkable base of biological data, high-throughput experimental tools will be required to assist in a practical and useful understanding of gene function.
Specific Instrumentation Goals
- Approaches to determining DNA sequence more rapidly, accurately, and economically, particularly to increase current maximum read lengths at least 2.5 times to 2000 to 2500 bases.
- Instrumentation that integrates and more thoroughly automates DNA sequence determination (e.g., sample preparation, loading, and analysis) and data analysis. Priority will be placed on approaches that emphasize miniaturization and microfabrication.
- Approaches that (1) verify a previously determined DNA sequence's accuracy without having to redetermine its entire sequence and (2) provide economical error-checking and proofreading of newly determined DNA sequence.
- Tools that enable efficient comparison of a known DNA sequence with a related but previously undetermined DNA sequence.
- Techniques for determining the functions of large numbers of genes in parallel, particularly those that match the speed and volume of DNA sequence determination.
This solicitation was intended to stimulate research that tests the applicability of concepts unrelated to the standard instrumentation for gene sequencing. The emphasis is on basic science that will enable genomic studies in the next century, when genomic data will be widely available and the appetite for new data will be undiminished. Robust tools for using this information within new quantitative, mechanistic, and predictive biology will be paramount. Work supported within the redirected program will hasten the arrival of an epoch when ideas and experiments requiring genome-scale data are within the scope of investigator-initiated, hypothesis-driven science.[Charles G. Edmonds (301/903-0042, Fax: -0567,charles.edmonds@oer.doe.gov]
Note:
The solicitation was the topic of an editorial in the May 1 issue of Analytical Chemistry [70(9), 292A], in which Royce Murray (University of North Carolina, Chapel Hill) urged readers to think creatively about the role analytical chemists might play in fulfilling OBER goals.
For a complete description of DOE's genome instrumentation goals, see http://www.science.doe.gov/grants/ [Program Notice 98-16 and companion Notice LAB98-16 (may be listed under Closed Solicitation Notices)].
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The electronic form of the newsletter may be cited in the following
style:
Human Genome Program, U.S. Department of Energy,
Human Genome News (v9n3).
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