This ultra-fast line scanning system is designed for live samples requiring either fast acquisition or high sensitivity. An auxiliary point scanner
allows for excitation of custom regions of interest. Photoactivation, FRAP and FRET of fluorescent proteins are fully supported. Heated perfusion
and solution change is available. A full environmental chamber will be installed in Spring 08.
Microscope stand:
- Axio Observer Z1 (inverted)
- Motorized XY stage
- Piezo stage focusing
- High-intensity, long-life X-cite light source
- FITC, Rhod and Triple (Dapi/FITC/Rhod) cubes
Objectives:
- 10x air EC Plan Neofluar 0.3 NA DIC M27
- 20x air Plan Apochromat 0.8 NA DIC M27
- 40x oil EC Plan Neofluar 1.3 NA DIC M27
- 63x oil Plan Apochromat 1.4 NA DIC M27 (piezo-mounted)
Laser lines (imaging only):
- 405 nm 50mW diode
- 488 nm 100mW diode
- 561 nm 40mW DPSS
- 647 nm 35mW diode
Filters:
- Steering channel 1/2:
- NFT 490
- NFT 510
- NFT 515
- NFT 565
- NFT 635
- Channel 1:
- LP 420
- BP 445-525
- LP 505
- BP 532-587
- BP 675-615 + LP 655
- LP 575
- BP 665-750 IR 0 deg
- BP 665-750 IR 90 deg
- Channel 2:
- BP 415-480
- BP 420-475 + 500-545 IR
- BP 445-525
- BP 470-500
- BP 495-555
- BP 505-610 IR
Detectors:
- Dual 512 pixels linear CCD, 80% quantum efficiency
- True 1024 and 1536 pixel resolution (scan shift)
- Dual-band emission filters for triple channel acquisition
- High-speed DIC imaging
DuoScan module:
- 405, 488 and 561 lines (635 nm not available)
- T80 / R20 and R 405 / 532 beam combiners
- Custom ROIs via point scanning
- Simultaneous bleaching / imaging
Software:
- Zeiss LSM v. 4.2
- Time serie
- FRAP, photoactivation
- Multitime, FRET macros
Microincubation:
- PDMI and DeltaT stage insert available
- Objective heater
- 5-channel heated perfusion
- Environmental chamber coming in 08 (CO2, temperature, humidity)
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