This point scanning instrument is available for either fixed slides or live specimen with temperature control, perfusion and solution changes. Photoactivation (405 nm), FRAP and FRET are fully supported. A Meta detector allows for spectral imaging and online emission fingerprinting. |
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Microscope |
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Zeiss Axiovert 200 M microscope (inverted) |
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Objectives |
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10x Zeiss plan-neofluar dry, 0.3 NA |
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20x Zeiss plan-apochromat dry, 0.75 NA |
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40x Zeiss plan-neofluar oil, 1.3 NA, DIC |
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63X Zeiss plan-apochromat oil, 1.4 NA, DIC |
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100x Zeiss alpha plan fluar oil, 1.45 NA, DIC |
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Fluorescence Filters Cubes (visual inspection only) |
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FITC / GFP: 450-490 nm excitation, 515-586 nm emission. |
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Rhodamin: 540-552 nm excitation, emission beyond 590 nm. |
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DAPI: 350-400 nm excitation, 417-477 nm emission. |
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Cy5: 610-650 nm excitation, 670-720 nm emission. |
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Laser Lines (scanning only) |
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405 nm: solid-state laser, 25 mW |
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458, 488 and 514 nm: Argon ion laser, 30 mW |
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543 nm: Helium-Neon laser, 1mW |
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633 nm: Helium-Neon laser, 15mW |
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AOTF switched: photoactivation, FRAP, FRET and uncagging fully supported |
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Filter Blocks (scanning only, must be used in certain combinations) |
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Excitation: |
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NT 80/20: beam splitter, 20% excitation reflected, 80% emission transmitted |
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HFT 405/488/543/633: dichroic 405, 488, 543 and 633 nm |
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HFT 405/488/543: dichroic 405, 488 and 543 nm |
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HFT 405/514: dichroic 405, 514 nm |
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HFT 458/514: dichroic 458, 514 nm |
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HFT 458/543: dichroic 458, 543 nm |
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HFT 458: dichroic 458 nm |
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HFT 488: dichroic 488 nm |
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Steering Meta vs PMT2/3: |
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NFT 490: long pass 490 nm |
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NFT 515: long pass 515 nm |
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NFT 545: long pass 545 nm |
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NFT 635 vis: long pass 635 nm |
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NFT KP 545: short pass 545 nm |
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Plate: glass plate correcting for misalignment, automatically inserted by software |
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Steering PMT 2 vs. PMT 3: |
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NFT 490: long pass 490 nm |
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NFT 515: long pass 515 nm |
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NFT 545: long pass 545 nm |
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Emission Filters |
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Meta detector |
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The Meta is a spectrometer spreading fluorescence emission among an array of detectors. It is, in itself, a configurable emission filter. |
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PMT 2 |
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LP 420: long pass 420 nm |
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LP 475: long pass 475 nm |
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LP 505: long pass 505 nm |
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BP 420-480 IR: band pass 420 to 480 nm, IR-blocking |
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BP 470-500: band pass 470 to 500 nm |
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BP 475-525: band pass 475 to 525 nm |
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BP 505-530: band pass 505 to 530 nm |
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PMT 3 |
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LP 505: long pass 505 nm |
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LP 560: long pass 560 nm |
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LP 650: long pass 650 nm |
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BP 500/20 IR: band pass 490 to 510 nm, IR-blocking |
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BP 505-530: band pass 505 to 530 nm |
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BP 505-550: band pass 505 to 550 nm |
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BP 530-600: band pass 530 to 600 nm |
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BP 560-615: band pass 560 to 615 nm |
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Detectors |
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Two photo-multiplier tubes fluorescence, 8 or 12 bit sampling (256 or 4096 gray levels, respectively) |
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One transmission detector for transmission / DIC imaging |
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One Meta detector for spectral imaging.. Up to 8 channels may be defined, each grouping any of the 32 detectors with a spectral width of 10 nm each (overlap between channels is not allowed). The meta detector is almost as sensitive as a PMT except at higher wavelength. However, its intrinsic noise is twice as high as a PMT. |
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Computer & Software |
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Fujitsu Siemens 2.4 GHz Xenon CPU running Windows 2000, 4 GB RAM, dual 114 GB storage, 2.4x DVD+/- recorder, dual 19" 1280 x 1024 flat panels |
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LSM 5 acquisition software with Physiology and Time Series modules |
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