Listed below are terms commonly used throughout this website.
Term |
Definition |
| GFP |
Green Flourescent Protein |
| S1-10 |
Strand 1 through 10 of the native GFP. LANL has engineered GFP so that it can be fragmented and each of the fragments remain soluble. |
| S11 |
Strand 11 of GFP. This is typically the "tether" or short fragment of GFP which is expressed as a tag to a protein of interest. |
| S1-9 |
Strand 1 through 9 of native GFP. LANL has engineered a version of GFP to do protein-protein interaction studies. S1-9 is the fragment of GFP that serves as the detector. |
| S10 |
Strand 10 of an engineered GFP that works with protein-protein interaction studies. S10 is a protein tag that when combined with the S1-9 detector, and the S11 tag attached to another protein, reconstitutes a functional GFP. |
| SuperFolder |
LANL engineered GFP which quantifies the expression level of a target protein, regardless of that protein's solubility. |
| GFP Tether, Tag |
This typically refers to the short fragment of GFP that is attached to a target protein. This fragment is only 14 amino acids long and is non-perturbing of the target protein's dynamics. |
| Aggregation |
This is the result of insoluble proteins "clumping" together. |
| GFP Detector |
This is the longest fragment of LANL's engineered GFP. It is used to determine if the "tether" or "tag" attached to a protein of interest is accessible (soluble). |
| Insertion Reporter |
LANL's version of engineered GFP that folds and fluoresces in a manner that indicates how well the target protein folds. |
| Split GFP |
A highly engineered version of GFP that is comprised of two independent and soluble fragments that recombine. |
| Passenger, target protein |
A protein of interest that is expressed with an attached GFP tag. |
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