Using LANL's GFP to Monitor Protein Expression (SuperFolder)

Overview

A highly engineered version of Green Fluorescent Protein (GFP) created by Los Alamos National Laboratory researcher, Dr. Waldo, can monitor the expression level of a target protein. Named "SuperFolder," this engineered GFP quantifies the expression level of a target protein because the amount of fluorescence is directly correlated with the amount of expressed protein. SuperFolder makes this possible because it folds and fluoresces no matter how poorly the target protein is expressed or how insoluble it is. If there is no fluorescence using SuperFolder, the researcher can safely conclude that the protein is not being expressed. Therefore, SuperFolder is a powerful tool to quickly and easily measure protein expression with a high level of confidence.

Key Characteristics

• Robust
• Reliable
• Quantifiable
• Fast and easy

Find more information about SuperFolder in our technical library.

RELATED LINKS

• Technical Library (pubs and presentations)
• GFP Wiki that defines common terms
• Feedback from our users
• Obtaining materials through MTAs