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Laboratory of Applied Mass Spectrometry (AMS)
Henry M Fales, PhD, Principal Investigator
The Laboratory specializes in the mass spectrometric analysis of peptides and proteins including all of the
chromatographic and specialized handling associated with that method. Currently, post-translational
modifications such as phosphorylation and glycosylation are a particular effort. Expertise is maintained
in the organic synthesis of reagents useful in characterizing peptides and proteins using mass spectrometry.
Selected Publications:
Boja, E.S., Hoodbhoy, T. Fales, H.M. Dean, J.(2003) Structural characterization of native mouse zona
pellucida proteins using mass spectrometry. J. Biol. Chem., 278(36):34189-34202
Zhao, M. Boja, E.S., Hoodbhoy, T., Nawrocki, J., Kaufman, J.B., Kresge, N, Ghirlando, R., Shiloach, J.,
Pannell, L., Levine, R.L., Fales, H.M., Dean, J.M., (2004) Mass Spectrometry Analysis of Recombinant
Human ZP3 Expressed in Glycosylation-Deficient CHO Cells. Biochemistry 43:12090-12104
Boja, E.S., Sokoloski, E.A., Fales, H.M. (2004) Divinyl sulfone as a postdigestion modifier for
enhancing the a(1) ion in MS/MS and postsource decay: potential applications in proteomics. Anal.
Chem. 76:3958-3970
Davis, D., Singer, K., De La Luz Sierra, M., Narazaki, M. Yang,F., Fales, H.M., Yarchoan, R.,
Tosato, G. (2005). Identification of Carboxypeptidase N as the plasma enzyme responsible for
C-terminal cleavage of stromal cell-derived factor-1 alpha. Blood 105, 4561-4568
Boja, E. S., Hoodbhoy, T., Garfield, M. Fales, H.M. (2005) Structural Conservation of Mouse
and Rat Zona Pellucida Glycoproteins. Probing the Native Rat Zona Pellucida Proteome by
Mass Spectrometry. (2005) Biochemistry, 44 16445-16460
Questions, comments and suggestions about this page may be addressed to
Henry M Fales
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