Sampling and Analytical Procedures for GLNPO's Open Lake Water Quality Survey of the Great Lakes

Prepared by:

Great Lakes National Program Office
U.S. Environmental Protection Agency
77 West Jackson Boulevard
Chicago, IL 60604

EPA 905-R-05-001
March 2010

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Chapter 1: Water Quality Survey Overview

Introduction (PDF) (6 pp.123 kb)
Scientific Check List (PDF) (2 pp.26 kb)
Standard Operating Procedure for General Shipboard Scientific Operations, Revision 04 (PDF) (14 pp.135 kb)
Standard Operating Procedure for Electronic Field Information Recording Revision 03 (PDF) (11 pp. 72 kb)

Chapter 2: Nutrient Parameters

Introduction (PDF) (4 pp.45 kb)
Field Sampling Using the Rosette Sampler, Revisions 06 (PDF) (12 pp.90 kb)
Standard Operating Procedure for Niskin Bottle Hydrographic Line Sampling Revision 01 (PDF) (6 pp.28 kb)
Standard Operating Procedure for Nitrate-Nitrite in Lake Water (QuikChem FIA+8000 Method) Revision 06 (PDF) (11 pp.98 kb)
Standard Operating Procedure for Total and Total Dissolved Phosphorous (Lachat Method 10-115-01-1F for QuikChem FIA+8000)Revision 07 (PDF) (12 pp. 209 kb)
Standard Operating Procedure for Chloride and Silica in Lake Water (Lachat Method), Revision 07 (PDF) (11 pp.144 kb)
Standard Operating Procedure for Sampling of Particulate-Phase Organic Carbon, Nitrogen and Phosporous in Great Lakes Waters, Revision 03 (PDF) (6 pp.1.2 Mb)
Standard Operating Procedure for the Analysis of Particulate-Phase Organic Carbon, Revision 03 (PDF) (13 pp.225 kb)
Standard Operating Procedure for Particulate-Phase Total Nitrogen by Alkaline Persulfate Oxidation Digestion (Lachat Method), Revision 06 (PDF) (8 pp.260 kb)
Standard Operating Procedure for Particulate-Phase Total Phosphorous by Persulfate Oxidation Digestion (Lachat Method), Revision 01 (PDF) (8 pp.245 kb)
Standard Operating Procedure for Sampling of Particulate-Phase and Dissolved-Phase Organic Carbon in Great Lakes Waters, Revision 06 (PDF) (7 pp.117 kb)
Standard Operating Procedure for Dissolved Organic Carbon, Revision 03 (PDF) (7 pp.138 kb)
Standard Operating Procedure for Nutrient Samples Processing, Revision 04 (PDF) (7 pp.105 kb)
Standard Operating Procedure for The Analysis of Metals in Waters and Wastewaters by ICP Method 200.7 Using the Perkin Elmer Optima 3300 DV and 43 DV, Original (PDF) (29 pp.439 kb)

Chapter 3: Physical Parameters

I ntroduction (PDF) (3 pp.53 kb)
Standard Operating Procedure for Meteorological Data Aboard ther R/V Peter Wise Lake Guardian, Revision 02 (PDF) (7 pp.42 kb
Operating the Seabird CTD, Original (PDF) (5 pp.33 kb)
Standard Operating Procedure for the Sampling and Analysis of Total Suspended Solids, Revision 04 (PDF) (10 pp.142 kb)
Standard Operating Procedure for the Determination of Dissolved O₂Correction Factors, Revision 01 (PDF) (7 pp.48 kb)
Standard Operating Procedure for Determination of Dissolved Oxygen in Lake Erie (See Appendix D) (PDF) (6 pp.14 kb)

Chapter 4: Biological Parameters

Introduction (PDF) (6 pp.39 kb)
Standard Operating Procedure for Phytoplankton Sample Collection and Preservation Field Procedures, Revision 09 (PDF) (7 pp.50 kb)
Standard Operating Procedure for Phytoplankton Analysis, Revisions 04 (PDF) (46 pp.1.6 mb)
Standard Operating Procedure for Zooplankton Sample Collection and Preservation and Secchi Depth Measurement Field Procedures, Revision 10 (PDF) (9 pp.163 kb)
Standard Operating Procedure for Zooplankton Analysis, Revision 03 (PDF) (20 pp.281 kb)
Standard Operating Procedure for Chlorophyll a Sampling Method Field Procedure, Revision 06 (PDF) (7 pp.59 kb)
Standard Operating Procedure for In Vitro Determination of Chlorophyll a in Freshwater Phytoplankton by Fluorescence, Revision 07 (PDF) (12 pp.244 kb)
Standard Operating Procedure for Benthic Invertebrate Field Sampling Procedure, Revision 07 (PDF) (9 pp.118 kb)
Benthic Invertebrate Laboratory Procedure, Revision 06 (PDF) (14 pp.104 kb)

Chapter 5: Board Chemistry Parameters

Introduction (PDF) (3 pp.40 kb)
Standard Operating Procedure for GLNPO Board Analysis, Revisions 07 (PDF) (15 pp.107 kb)
Standard Operating Procedure for Dissolved Oxygen Micro Method, Winkler Titration, Revisions 06 (PDF) (8 pp.266 kb)
Standard Operating Procedure for Total Hardness (PDF) (5 pp.33 kb)

Chapter 6: Nutrients in Sediments

Introduction (PDF) (6 pp.39 kb)
Standard Operating Procedure for Total Phosphorous in Sediments by Persulfate Oxidation Digestion (Lachat Method), Revision 03 (PDF) (6 pp.152 kb)
Standard Operating Procedure for Analysis of Total Organic Carbon in Sediments (Dry Combustion, IR Detection), Revision 04 (PDF) (6 pp.114 kb)
Standard Operating Procedure for Total Nitrogen in Sediments by Alkaline Persulfate Oxidation Digestions (Lachat Method), Revision 04 (PDF) (2 pp.232 kb)

Appendices:

Appendix A    Great Lakes Maps of Survey Stations (PDF) (3 pp.9kb)
Appendix B    Quality Assurance Project Plan (QAPP) for the Great Lakes Water Quality Surveys Revision 02 (PDF) (106 pp.939 kb)
Appendix C    Great Lakes Water Quality/Limnology Survey Staffing Schedule "Original" (PDF) (2 pp.23 kb)
Appendix D    Dissolved Oxygen and Temperature Profiles for the Central Basin of Lake Erie Quality Assurance Project Plan (QAPP) Revision 5 (PDF) (77 pp.540 kb)
Appendix E    Survey Plan Submittal Forms Revison 02 (PDF) (15 pp.152 kb)
Appendix F    Self-certification Form Revison 01 (PDF) (2 pp.22 kb)
Appendix G    Medical History Questionaire Revision 01 (PDF) (4 pp.33 kb)
Appendix H    Hard-copy Field Information Recording Forms Revision 05 (PDF) (3 pp.257 kb)
Appendix I    Lists of Acceptable GLENDA Codes Version 1.04 and 1.05 (PDF) (18 pp.65 kb)
Appendix J    User's Manual for the Remote Data Entry Tool Version 3.1 (PDF) (31 pp.479 kb)
Appendix K    GLENDA Analyte Names and Codes Version 1.16 (PDF) (34 pp.78 kb)
Appendix L    Chief Scientist Roles and Responsibilities, and List of Priorities for Each Staffing Period and Chief Scientist
                      and Shift Supervision List of Responsibilities and Checklist for Each Shift Revision 02 (PDF) (4 pp.45 kb)
Appendix M    Issues and Revisions Sheet "Original" (PDF) (19 pp.29 kb)
Appendix N    Data Status Tracking Sheet "Original" (PDF) (19 pp.986 kb)
Appendix O    Data Discrepancy Form "Original" (PDF) (3 pp.32 kb)
Appendix P    Summary of Sample Depths Collected as part of the WQA for all Five Lakes Revision 01 (PDF) (7 pp.85 kb)
Appendix Q    Station Location Change Form "Original" (PDF) (3 pp.23 kb)
Appendix R    Winter Operations Revision 01 (PDF) (3 pp.23 kb)