Genome Project 
U.S. HGP on Fast Track 
DOE Joint Genome Institute Exceeds Goal 
New 5-Year Goals 
Faster Sequencing with BACs 
Mapping with STCs and STSs 
Availability of BAC Clones and STC Data 
BAC Related Websites 
BAC Resource Success Story 
Scientists Hunt SNPs for Variation, Disease 
Who's Sequencing the Human Genome? 
Genomics Progress in Science 
EMSL Promotes Remote Access to Instrumentation 
Second Private-Sector Sequencing Project 
GeneMap'98 

In the News 
Team Delivers C. elegans Sequence 
Why Sequence Entire Genomes? Worm's Eye View 
Embnet.news on Web 
European Biotech Program 
DOE BER Research Update 
Hollaender Fellows Named 
SBIR 1998 Human Genome Awards Announced 
Mouse Resources 
Mouse Consortium for Functional Genomics 
Chlamydia Genome Analysis 
HUGO Merges Offices, Web Sites 

Microbial Genomics 
Superbug Deinococcus radiodurans 
Unfinished Microbial Genomes Searchable 
TIGR Releases Chlorobium tepidum Sequence 
DOE MGP Abstracts Online 
Microbial TV Series 
 
Ethical, Legal, and Social Issues and Educational Resources 
Cambridge ELSI Symposium 
Eric Lander, Genetics in the 21st Century 
Mark Rothstein, Genetic Privacy 
James Wilson, Gene Therapy Present & Future 
LeRoy Walters, Ethical Issues in Gene Therapy 
DNA Files on NPR, Internet 
Innovative Biotechnology Curriculum 
Short Course for Biology Teachers 
Microbial TV Series 

Proteomics 
Looking at Proteins to Understand Expression 
2-DGE:  Protein Visualization, Modification 
Tool for Protein Analysis 
TREMBL Release 6 
R&D 100 Award Goes to LANL's SOLVE 
NIH Awards Proteomics Grant to Axys 
E. coli Proteome Database 

Genetics in Medicine 
National Organization for Rare Disorders 
Translation of Genetics to Medicine: New Website 
Cancer Genetics Web Site 
HuGem Website Offers Education in New Genetics 
Calculation of Genetic Risks 2nd Edition 
New Genetics Manual Offered 
Mutation Research Genomics Online 
 
Informatics 
GDB Database Operations Restored 
In Silico Biology: Bioinformatics Journal 
Computational Methods Book Available 
Bioinformatics Guide
BioToolKit
Gene-Finding Programs at Sanger
New Sequin Version
Tandem Repeat Tool
Sequence Viewer
SmithKline Licenses Gene Logic Software 
Influenza Database at LANL 
TRANSFAC Database 
p53 Mutation Database 
TBASE at Jackson Laboratory 
Intein Database on Web 
System Identifies Polymorphisms 

Web, Other Resources, Publications 
1999 Oakland Workshop Website 
Launchpad to Human Chromosomes 
Nature Genetics Supplement 

Funding 
DOE Office of Science Grants and Contracts 
NHGRI National Service Award Fellowships 
NCI Technologies for Molecular Analysis 
NIH: Netork for Large-Scale Mouse Sequencing 
NHGRI: Genomic Technology Development 
US Genome Research Funding 

Meeting Calendars & Acronyms 
Genome and Biotechnology Meetings 
Training Courses and Workshops 
Acronyms 

HGN archives and subscriptions 
HGP Information home

Mouse Resources Critical to Understanding Human Genome

Some 60 scientists met for 3 days in March 1998 in Bethesda, Maryland, to define priorities for producing resources to make the mouse a more valuable tool for understanding mammalian biology. Convened by NIH Director Harold Varmus, the Mouse Genomics and Genetics Resources Working Group's recommendations, as summarized by cochairs William Dove (University of Wisconsin) and David Cox (Stanford University), are outlined below. Total direct costs for the first year are estimated at $49.3 million.

The first follow-up meeting was held in October 1998 to discuss implementation of the March recommendations. Representatives from DOE and the U.K.'s Medical Research Council were present to develop a coordinated strategy and share expertise in this international effort.

Recommendations

Structural Analysis

• Generate an additional 60,000 new markers, identified as crucial for scientists who are cloning genes.
• Genotype inbred mouse strains and generate a low-resolution (5-cM) single-nucleotide polymorphism map to determine its value for mouse research.
• Sequence and map 3' ends of partial cDNAs and improve methods for isolating missing and full-length cDNAs.
• Generate 12 Mb of sequence for the first year and ramp up to 400 Mb within 5 years, obtaining a completed reference mouse genomic sequence by 2008.

• Develop standardized genome-wide mutagenesis protocols and improved tools and assays for characterizing phenotypes within new, specialized centers using the supermutagen ENU (ethyl nitrosourea, developed at Oak Ridge National Laboratory by William Russell).
• Develop phenotyping protocols in ENU centers and by individual investigators.
• Set up targeted mutagenesis programs to validate embryonic stem lines from different mouse strains for specialized uses.
• Couple molecular genotyping with the construction of congenic mouse strains.

• Develop cryopreservation methods and facilities for maintaining mutant mouse sperm and ovaries, thus reducing the cost of maintaining live animals.
• Build a new repository for live mouse strains.
• Evaluate and expand some existing databases.
• Train researchers in cryopreservation technology and animal pathology.

• March meeting
• NIH action plan
• October meeting
• Mouse Genome Sequencing Network RFA

The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v10n1-2).