Projects

APPLICATION OF BIOLOGICAL AND MOLECULAR TECHNIQUES TO THE DIAGNOSIS AND CONTROL OF AVIAN INFLUENZA AND OTHER EMERGING POULTRY PATHOGENS Appropriated (D)   Accession Number: 411346

NEWCASTLE DISEASE EPIDEMIOLOGY, PATHOGENESIS, AND CONTROL Appropriated (D)   Accession Number: 411482

DEVELOPMENT OF REAGENTS AND PROTOCOLS FOR DIAGNOSTICS AND IMAGING OF NEWCASTLE DISEASE VIRUS Nonfunded Cooperative Agreement (N)   Accession Number: 411472

DEVELOPMENT OF ASSAYS FOR IDENTIFICATION OF NEWLY EVOLVED VIRULENT STRAINS OF NEWCASTLE DISEASE VIRUS. Trust (T)   Accession Number: 413363

EMERGENCY VETERINARY MEDICAL RESEARCH ON VACCINES, ENVIRON. SURVEILLANCE, GENOMICS & BIOSECURITY FOR H5N1 HIGH PATHOGENICITY AVIAN INFLUENZA Other (X)   Accession Number: 410283

Publications

Role of host gene expression on infection, disease and lethality of HPAI virus infection in ducks - (Abstract) - (28-Jan-09)

Use of genomic interspecies microarray hybridization to detect differentially expressed genes associated with H5N1 avian influenza virus infections in ducks - (Abstract) - (21-Aug-08)

Biological and phylogenetic characterization of pigeon paramyxovirus serotype-1 circulating in North American pigeons - (Peer Reviewed Journal)

Kim, L.M., King, D.J., Guzman, H., Tesh, R.B., Bueno, R., Dennett, J.A., Afonso, C.L. 2008. Biological and phylogenetic characterization of pigeon paramyxovirus serotype-1 circulating in North American pigeons and doves. Journal of Clinical Microbiology. 46(10):3303-3310.

Not so fast on recombination analysis of Newcastle disease virus - (Other)

Afonso, C.L. 2008. Not so fast on recombination analysis of Newcastle disease virus. Journal of Virology. 82(18):9303.

Full genome sequencing of the Newcastle disease viruses VS/GA and clone 5 - (Peer Reviewed Journal)

Perozo, F., Villegas, P., Afonso, C.L. 2008. Genomic comparison of the complete coding and intergenic regions of the VG/GA Newcastle disease virus and its respirotropic clone 5. Virus Genes. 37(2):161-167.

Highly pathogenic H5N1 avian influenza viruses differentially affect gene expression in primary chicken embryo fibroblasts - (Peer Reviewed Journal) - (13-May-08)

Biological and phylogenic characterization of virulent Newcastle disease virus circulating in Mexico - (Peer Reviewed Journal) - (08-May-08)

Microarray analysis following infection with highly pathogenic avian influenza H5N1 virus in naive and vaccinated SPF chickens - (Abstract)

Kapczynski, D.R., Afonso, C.L., Pantin Jackwood, M.J., Liljebjelke, K.A., Sarmento, L., Suarez, D.L., Swayne, D.E. 2008. Microarray analysis following infection with highly pathogenic avian influenza H5N1 virus in naive and vaccinated SPF chickens [abstract]. In: Proceedings of Avian Immunology Research Group Conference, June 24-27, 2008, Queensland, Australia. p. 22.

Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real time RT-PCR assay - (Peer Reviewed Journal)

Kim, L.M., Suarez, D.L., Afonso, C.L. 2008. Detection of a broad range of class I and II Newcastle disease viruses using a multiplex real-time reverse transcription polymerase chain reaction assay. Journal of Veterinary Diagnostic Investigation. 20:414-425.

The VG/GA strain of Newcastle disease virus: Mucosal immunity, protection against lethal challenge and molecular analysis - (Peer Reviewed Journal)

Perozo, F., Villegas, P., Dolz, R., Afonso, C.L., Purvis, L.B. 2008. The VG/GA strain of Newcastle disease virus: Mucosal immunity, protection against lethal challenge and molecular analysis. Avian Pathology. 37(3):237-245.

Phylogenetic and biological characterization of virulent Newcastle disease viruses isolated in wild birds during 2002-2007 - (Abstract)

Afonso, C.L., King, D.J., Kim, L.M., Guzman, H., Tesh, R.B., Dennett, J.A. 2000. Phylogenetic and biological characterization of virulent Newcastle disease viruses isolated in wild birds during 2002-2007 [abstract]. In: Proceedings of the American Association of Avian Pathologists meeting, July 19-23, 2008, New Orleans, Louisiana. p. 118.

Gene expression responses to highly pathogenic avian influenza H5N1 virus infections in ducks - (Abstract)

Pantin Jackwood, M.J., Kapczynski, D.R., Wasilenko, J.L., Sarmento, L., Afonso, C.L. 2008. Gene expression responses to highly pathogenic avian influenza H5N1 virus infections in ducks [abstract]. 145th American Medical Association Annual Meeting, July 20-23, 2008, New Orleans, Louisiana. 2008 CDROM.

Viral genome sequencing bt random priming methods - (Peer Reviewed Journal)

Djikeng, A., Halpin, R., Kuzmickas, R., Sengamalay, N., Afonso, C.L., Xang, X., Anderson, N.G., Ghedin, E., Spiro, D.J. 2008. Viral genome sequencing by random priming methods. Biomed Central (BMC) Genomics. 9:5.

Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian invluenza virus - (Peer Reviewed Journal)

Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E., Afonso, C.L. 2008. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian invluenza virus. Biologicals. 132:175-183.

Baculoviruses: Molecular Biology of Mosquito Baculoviruses - (Peer Reviewed Journal)

Becnel, J.J., Afonso, C.L. 2008. Baculoviruses: Molecular Biology of Mosquito Baculoviruses. Encyclopedia of virology, 5 vols. (B.W.J. Mahy and M.H.V. Van Regenmortel, Editors), pp. 219-225 Oxford:Elsevier.

Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates - (Abstract)

Merino, R., Calderon, N., Perozo, F., Villegas, P., Afonso, C.L. 2008. Characterization and phylogenic analysis of Mexican Newcastle disease virus isolates [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 33.

Variable regulation of chicken embryo fibroblasts gene expression by H5N1 avian influenza viruses - (Abstract)

Sarmento, L., Pantin Jackwood, M.J., Afonso, C.L., Wasilenko, J.L., Estevez, C. 2008. Variable regulation of chicken embryo fibroblasts gene expression by H5N1 avian influenza viruses [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 33.

Primer sequence-independent full genome amplification and sequencing of the VG/GA strain of Newcastle disease virus - (Abstract)

Perozo, F., Villegas, P., Afonso, C.L. 2008. Primer sequence-independent full genome amplification and sequencing of the VG/GA strain of Newcastle disease virus [abstract]. International Poultry Scientific Forum, January 21-22, 2008, Atlanta, Georgia. p. 32.

Research update on exotic and emerging poultry diseases - (Proceedings/Symposium)

Pantin Jackwood, M.J., Suarez, D.L., Zsak, L., Spackman, E., Kapczynski, D.R., King, D.J., Afonso, C.L., Day, J.M., Spatz, S.J., Yu, Q., Swayne, D.E. 2007. Research update on exotic and emerging poultry diseases. In: Proceedings of the 111th Annual Meeting of the U. S. Animal Health Association, Reno, Nevada, October 22-23, 2007. p. 702-707. Available: http://www.usaha.org/meetings/2007/2007_USAHA_Proceedings.pdf.

A primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses - (Peer Reviewed Journal)

Zaffuto, K.M., Estevez, C., Afonso, C.L. 2008. Primary chicken tracheal cell culture system for the study of infection with avian respiratory viruses. Avian Pathology. 37(1):25-31.

Phylogenetic diversity among low virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to poultry-origin isolates - (Peer Reviewed Journal)

Kim, L.M., King, D.J., Curry, P., Suarez, D.L., Swayne, D.E., Stallknecht, D., Slemons, R.D., Pedersen, J.C., Senne, D.S., Winker, K., Afonso, C.L. 2007. Phylogenetic diversity among low-virulence Newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates. Journal of Virology. 81(22):12641-12653.

Sheeppox virus kelch-like gene SPPV-019 affects virus virulence - (Peer Reviewed Journal)

Balinsky, C.A., Delhon, G., Afonso, C.L., Risatti, G.R., Borca, M.V., French, R.A., Tulman, E.R., Geary, S.J., Rock, D.L. 2007. Sheeppox virus kelch-like gene SPPV-019 affects virus virulence. Journal of Virology. 81(20):11392-11401.

Antigenic differences among NDV strains of different genotypes used in vaccine formulation affects viral shedding after a virulent challenge - (Peer Reviewed Journal)

Miller, P.J., King, D.J., Afonso, C.L., Suarez, D.L. 2007. Antigenic differences among NDV strains of different genotypes used in vaccine formulation affects viral shedding after a virulent challenge. Vaccine. 25:7238-7246.

Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages - (Peer Reviewed Journal)

Zaffuto, K.M., Piccone, M.E., Burrage, T.G., Risatti, G.R., Borca, M.V., Holinka, L.G., Rock, D.L., Afonso, C.L. 2007. Classical Swine Fever Virus Inhibits Nitric Oxide Production in Infected Macrophages. Journal of General Virology. 88:3007-3012.

Early responses of chicken lungs and spleens to infection with highly pathogenic avian influenza virus using microarray analysis - (Abstract)

Afonso, C.L., Kapczynski, D.R., Pantin Jackwood, M.J., Sarmento, L., Swayne, D.E. 2007. Early responses of chicken lungs and spleens to infection with highly pathogenic avian influenza virus using microarray analysis [abstract]. In: Proceedings of the International Symposium on Animal Genomics for Animal Health, October 23-25, 2007, Paris France. p. 59.

Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza - (Abstract)

Afonso, C.L., Sarmento, L., Pantin Jackwood, M.J., Kapczynski, D.R., Swayne, D.E. 2007. Immediate early responses of avian tracheal epithelial cells to infection with highly pathogenic avian influenza [abstract]. In: Proceedings of the International Symposium on Animal Genomics for Animal Health, October 23-25, 2007, Paris, France. p. 16.

Detection and isolation of exotic Newcastle disease (ENDV) from field collected flies - (Peer Reviewed Journal)

Chakrabarti, S., King, D.J., Afonso, C.L., Swayne, D.E., Cardona, C.J., Kuney, D.R., Gerry, A.C. 2007. Detection and isolation of exotic Newcastle disease virus from field-collected flies. Journal of Medical Entomology. 44(5):840-844.

Design of a multiplex real time RT-PCR assay to detect Newcastle disease viruses from classes I and II - (Abstract)

Kim, L.M., Suarez, D.L., Afonso, C.L. 2007. Design of a multiplex real time RT-PCR assay to detect Newcastle disease viruses from classes I and II [abstract]. 10th International Conference for Biodetection Technologies 2007, June 14-15, 2007, Atlanta, Georgia. Poster #8.

Sequencing of avian influenza virus genomes following random amplification - (Peer Reviewed Journal)

Afonso, C.L. 2007. Sequencing of avian influenza virus genomes following random amplification. Biotechniques. 43(2):188-192.

Isolation and genetic characterization of influenza A viruses and a paramyxovirus from wild birds in Barbados: 2003-2004 - (Peer Reviewed Journal)

Douglas, K.O., Lavoie, M.L., Suarez, D.L., Afonso, C.L., Kim, L.M. 2007. Isolation and genetic characterization of avian influenza viruses and a Newcastle disease virus from wild birds in Barbados: 2003-2004. Avian Diseases. 51(3):781-787.

Isolation of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species - (Abstract)

King, D.J., Chakrabarti, S., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2007. Isolation of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species [abstract]. American Association of Avian Pathologists/American Veterinary Medical Association Meeting Program, July 14-18, 2007, Washington, DC. p. 44.

Characterization of Class I Newcastle disease virus isolates from Hong Kong bird markets and detection using real-time reverse transcription PCR - (Peer Reviewed Journal)

Kim, L.M., King, D.J., Suarez, D.L., Wong, C.W., Afonso, C.L. 2007. Characterization of Class I Newcastle disease virus isolates from Hong Kong bird markets and detection using real-time reverse transcription PCR. Journal of Clinical Microbiology. 45:1310-1314.

Newcastle disease virus isolates from US waterfowl reveal novel genomic subgroups with diverse and evolving genetics and the potential for spillover into US live bird markets - (Abstract)

Kim, L.M., King, D.J., Swayne, D.E., Stallknecht, D., Slemons, R.D., Pedersen, J.C., Senne, D.A., Winker, K., Afonso, C.L. 2007. Newcastle disease virus isolates from US waterfowl reveal novel genomic subgroups with diverse and evolving genetics and the potential for spillover into US live bird markets [abstract]. In: Abstracts of the American Association of Avian Pathologists meeting, July 14-19, 2007, Washington, DC. p. 43. CD.

Development and characterization of a primary culture of chicken embryonic tracheal epithelial cells and their use in avian studies - (Abstract)

Zaffuto, K.M., Suarez, D.L., Estevez, C., Afonso, C.L. 2007. Development and characterization of a primary culture of chicken embryonic tracheal epithelial cells and their use in avian studies [abstract]. American Association of Avian Pathologists Annual Meeting, July 14-19, 2007, Washington, DC. p. 17.

Differential growth of avian influenza virus in chicken and duck cells - (Abstract)

Sarmento, L., Zaffuto, K.M., Pantin Jackwood, M.J., Afonso, C.L. 2007. Differential growth of avian influenza virus in chicken and duck cells [abstract]. 144th American Veterinary Medical Association Annual Meeting, July 14-19, 2007, Washington, DC. CD-ROM.

Why are avian influenza viruses emerging and what tools are needed to prevent and control the infection and disease? - (Abstract)

Swayne, D.E., Suarez, D.L., Pantin Jackwood, M.J., Spackman, E., Kapczynski, D.R., Afonso, C.L. 2007. Why are avian influenza viruses emerging and what tools are needed to prevent and control the infection and disease?. In: Proceedings of the 110th Annual Meeting of the U.S. Animal Health Association, October 12-18, 2006, Minneapolis, Minnesota. p. 728-729.

Use of bioinformatics in improving detection of Newcastle disease virus - (Book/Chapter)

Kim, L.M., Suarez, D.L., Afonso, C.L. 2007. Use of bioinformatics in improving detection of Newcastle disease virus. In: Rogeria de Almeida, M., Pires Moraes, M., Patarroyo, J.H., Vidigal, P.M.P., Borem, A., editors. Biotecnologia e Saude Animal. Viscoza, Brazil: Federal University of Viscosa. Chapter 9. p. 227-249.

Genus Suipoxvirus - (Book/Chapter)

Gustavo, D.A., Tulman, E.R., Afonso, C.L., Rock, D.L. 2007. Genus Suipoxvirus. In: Mercer, A., Schmidt, A., Weber, O. (editors). Poxviruses. Birkhauser Basel. p. 203-215.

RECOVERY OF EXOTIC NEWCASTLE DISEASE VIRUS (ENDV) FROM FIELD COLLECTED FLIES AND EXPERIMENTAL ENDV INFECTIONS OF THREE ARTHROPOD SPECIES - (Abstract)

Chakrabarti, S., King, D.J., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2006. Recovery of exotic Newcastle disease virus (ENDV) from field collected flies and experimental ENDV infections of three arthropod species [abstract]. The 54th Annual Meeting of the Entomological Society of America, December 10-13, 2006, Indianapolis, Indiana. p. 93.

GENOME OF INVERTEBRATE IRIDESCENT VIRUS 3 (MOSQUITO IRIDESCENT VIRUS) - (Peer Reviewed Journal)

Delhon, G., Tulman, E., Afonso, C.L., Lu, Z., Becnel, J.J., Moser, B.A., Kutish, G.F., Rock, D.L. 2006. Genome of invertebrate iridescent virus 3 (mosquito iridescent virus). Journal of Virology. 80(17):8439-8449.

GENOME OF HORSEPOX VIRUS - (Peer Reviewed Journal)

Tulman, E., Delhon, G., Afonso, C.L., Lu, Z., Zsak, L., Sandybaev, N.T., Kerembekova, U.Z., Zaitsev, V.L., Kutish, G.F., Rock, D.L. 2006. Genome of horsepox virus. Journal of Virology. 80(18):9244-9258.

POTENTIAL ROLE OF FLIES IN THE PERSISTENCE AND DISPERSAL OF EXOTIC NEWCASTLE DISEASE VIRUS IN SOUTHERN CALIFORNIA - (Abstract)

Chakrabarti, S., King, D.J., Cardona, C., Afonso, C.L., Swayne, D.E., Gerry, A.C. 2006. Potential role of flies in the persistence and dispersal of exotic Newcastle disease virus in Southern California [abstract]. Livestock Insect Worker's Conference Annual Meeting. p. 34.

PHYLOGENETIC CHARACTERIZATION OF ENDEMIC NEWCASTLE DISEASE VIRUSES ISOLATED FROM WILD BIRDS DURING 2000 TO 2004 IN DELAWARE, MARYLAND AND NEW JERSEY - (Abstract)

Afonso, C.L. 2006. Phylogenetic characterization of endemic Newcastle disease viruses isolated from wild birds during 2000 to 2004 in Delaware, Maryland and New Jersey [abstract]. American Association of Avian Pathologists 2006 Meeting, Hawaii. p. 154.

EFFECT OF PROBE-SITE MISMATCHES ON DETECTION OF VIRULENT NEWCASTLE DISEASE VIRUSES USING A FUSION-GENE REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION TEST - (Peer Reviewed Journal)

Kim, L.M., Afonso, C.L., Suarez, D.L. 2006. Effect of probe-site mismatches on detection of virulent Newcastle disease viruses using a fusion-gene real-time reverse transcription polymerase chain reaction test. Journal of Veterinary Diagnostic Investigation. 18:519-528.

REAL TIME RT-PCR DEVELOPED FOR A PHYLOGENETICALLY DIVERGENT GROUP OF NEWCASTLE VIRUSES NOT DETECTED BY CURRENT TESTS - (Abstract)

Kim, L.M., Afonso, C.L., Suarez, D.L., King, D.J. 2006. Real time RT-PCR developed for a phylogenetically divergent group of Newcastle viruses not detected by current tests [abstract]. American Association of Avian Pathologists. CD p. 156.

GENOME OF CROCODILEPOX VIRUS - (Peer Reviewed Journal)

Afonso, C.L., Tulman, E.R., Delhon, G., Lu, Z., Viljoen, G.J., Wallace, D.B., Kutish, G.F., Rock, D.L. 2006. Genome of crocodilepox virus. Journal of Virology. 80(10)4978-4991.

CONTRASTING RESULTS FROM MOLECULAR AND BIOLOGICAL PATHOGENICITY ASSAYS OF NEWCASTLE DISEASE VIRUS ISOLATE IOWA 1519 - (Abstract)

King, D.J., Kapczynski, D.R., Afonso, C.L., Piacenti, A. 2005. Contrasting results from molecular and biological pathogenicity assays of newcastle disease virus isolate Iowa 1519 [abstract]. American Association of Avian Pathologists, p.33.

GENOME SEQUENCING AND ANALYSIS - (Proceedings/Symposium)

Afonso, C.L., Kutish, G.F. 2004. Genome sequencing and analysis [abstract]. Society of Invertebrate Pathology.

AFRICAN SWINE FEVER VIRUS MULTIGENE FAMILY 360 AND 530 GENES AFFECT HOST INTERFERON RESPONSE - (Peer Reviewed Journal)

Afonso, C., Piccone, M.E., Zaffuto, K.M., Neilan, J.G., Kutish, G., Lu, Z., Balinsky, C.A., Gibb, T., Bean, T.R., Zsak, L., Rock, D.L. 2004. African swine fever virus multigene family 360 and 530 genes affect host interferon response.. Journal of Virology. (2004) 78 (4):1858-1864.

AFRICAN SWINE FEVER VIRUS MULTIGENE FAMILY 360 AND 530 GENES AFFECT HOST INTERFERON RESPONSE - (Peer Reviewed Journal)

Afonso, C.L., Piccone, M.E., Zaffuto, K., Neilan, J.G., Kutish, G.F., Lu, Z., Balinsky, C.A., Gibb, T.R., Zsak, L., Rock, D.L. 2004. African swine fever virus multigene family 360 and 530 genes affect host interferon response. Journal of Virology. 78(4):1858-1864.

GENOMES OF THE PARAPOXVIRUSES ORF VIRUS AND BOVINE PAPULAR STOMATITIS VIRUS - (Peer Reviewed Journal)

Delhon, G., Tulman, E., Afonso, C., Lu, Z., De La Concha-Bermeji, A., Lehmkuhl, H., Piccone, M.E., Kutish, G., Rock, D.L. 2004. Genomes of the parapoxviruses orf virus and bovine papular stomatitis virus.. Journal of Virology. 78(1):168-177.

GENOMES OF PARAPOXVIRUSES ORF VIRUS AND BOVINE PAPULAR STOMATITIS VIRUS - (Peer Reviewed Journal)

Delhon, G., Tulman, E., Afonso, C.L., Lu, Z., Concha-Bermejillo, A., Lehmkuhl, H., Piccone, M.E., Kutish, G.F., Rock, D.L. 2004. Genomes of parapoxviruses orf virus and bovine papular stomatitis virus. Journal of Virology. 78(1):168-177.

THE GENOME OF CANARYPOX VIRUS - (Peer Reviewed Journal)

Tulman, E., Afonso, C.L., Lu, Z., Zsak, L., Kutish, G.F., Rock, D.L. 2004. The genome of canarypox virus. Journal of Virology. 78(1):353-366.

HZ-2V GENOME ANALYSIS - (Proceedings/Symposium)

Kim, W., Burand, J.P., Afonso, C.L., Kutish, G.F., Lu, Z., Rock, D.L. 2003.Hz-2v genome analysis [abstract]. Society for Invertebrate Pathology Annual Meeting. P. 78.

GENOME OF BOVINE HERPESVIRUS 5 - (Peer Reviewed Journal)

Delhon, G., Moraes, M.P., Lu, Z., Afonso, C.L., Flores, E.F., Weiblen, R., Kutish, G.F., Rock, D.L. 2003. Genome of bovine herpesvirus 5. Journal of Virology. 77(19):10339-10347.

LIGATION PROPERTIES OF A NEW CLASS OF NAD-DEPENDENT DNA LIGASE FROM ENTOMOPOXVIRUS MELANOPLUS SANGUINIPES - (Peer Reviewed Journal) - (22-Mar-02)