Project Number: 6612-32000-049-04
Project Type: Trust

Start Date: Sep 01, 2008
End Date: Aug 30, 2009

Objective:
To evaluate the performance of current US NDV real time diagnostics assays against new isolates of NDV.

Approach:
1)The sensitivity and efficacy of the Matrix real time PCR test on 50 NDV isolates from diverse genotypes isolated worldwide will be tested using RT-PCR assays performed on RNA isolated from allantoic fluids under standard approved conditions. 2) The sensitivity and efficacy of the new SEPRL real time multiplex test on 50 NDV isolates from diverse genotypes isolated worldwide will be tested on RNA isolated from allantoic fluids using standard real time PCR approved conditions. 3) The sensitivity and efficacy of the fusion protein test will be tested on 50 NDV isolates using RNA isolated from allantoic fluids and standard approved real time conditions. 4) Sequencing of new isolates that are not detected with current methods will be done on PCR products obtained with NDV specific primers.