Bruce S Dien
Fermentation Biotechnology Research
Chemical Engineer
Phone: (309) 681-6270
Fax: (309) 681-6427
Room 3300 1815 N UNIVERSITY ST
PEORIA, IL, 61604-3999
Qureshi, N., Saha, B.C., Iten, L.B., Sarath, G., Dien, B.S., Cotta, M.A. 2008. Agricultural Residues and Energy Crops as Novel Substrates for Butanol Production by Fermentation [abstract]. In: Proceedings of 10th International Workshop and Conference on Regulation of Metabolism, Genetics, and Development of Solvent and Acid Forming Clostridia, September 28-October 1, 2008, Holland, The Netherlands. Paper No. 19.
Dien, B.S., Sarath, G., Pedersen, J.F., Funnell-Harris, D.L., Sattler, S.E., Nichols, N.N. 2008. Evaluating low lignin mutants of forage sorghum for increased conversion efficiency to sugars and ethanol. Biotechnology for Fuels and Chemicals. p. 267.
Dien, B.S., Sarath, G., Pedersen, J.F., Vogel, K.P., Jung, H.G., Sattler, S.E., Casler, M.D., Mitchell, R., Cotta, M.A. 2008. Energy crops for ethanol: a processing perspective. In: Proceedings of the 5th International Crops Science Congress, April 13-18, 2008, Jeju Island, Korea. p. 1-5.
Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2007. Developing herbaceous energy crops as feedstocks for biofuel production. In: Proceedings of the UJNR Food and Agricultural Panel, October 21-25, 2007, Tsukuba, Japan. p. 137-140.
Qureshi, N., Ezeji, T.C., Ebener, J., Dien, B.S., Cotta, M.A., Blaschek, H.P. 2008. Butanol production by Clostridium beijerinckii. Part I. Use of acid and enzyme hydrolysed corn fiber. Bioresource Technology. 99:5915-5922.
Dien, B.S., Ximenes, E.A., O'Bryan, P.J., Moniruzzaman, M., Li, X., Balan, V., Dale, B., Cotta, M.A. 2008. Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol. Bioresource Technology. 99(12):5216-5225.
Anderson, W.F., Dien, B.S., Brandon, S.K., Peterson, J. 2008. Assessment of bermudagrass and bunch grasses as feedstock for conversion to ethanol. Applied Biochemistry and Biotechnology. 145(1-3):13-21.
Srinivasan, R., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2007. Fiber Separated from Distillers Dried Grains with Solubles as a Feedstock for Ethanol Production. Cereal Chemistry. 84(6):563-566.
Dien, B.S. 2007. A primer for lignocellulose biochemical conversion to fuel ethanol. Proceedings of the Fifth International Starch Technology Conference. p. 5.1-5.9.
Bischoff, K.M., Liu, S., Dien, B.S. 2007. Monensin inhibits growth of bacterial contaminants from fuel ethanol plants [abstract]. American Society for Microbiology. Poster #0-010. p. 60.
Anderson, W.F., Dien, B.S., Peterson, J., Brandon, S.K., Akin, D.E. 2007. Assessment of bermudagrass as a feedstock for ethanol. [abstract]. ASA-CSSA-SSSA International Meetings, November 4-8, 2007, New Orleans, Louisiana. Cd Rom.
Dien, B.S., Cotta, M.A., Jung, H.G., Vogel, K.P. 2007. Developing herbaceous energy crops as feedstocks for bioethanol production [abstract]. American Chemical Society. Paper No. Agro-148.
Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol [abstract]. 233rd American Chemical Society National Meeting & Exposition, March 25-29, 2007, Chicago, IL. p. 107.
Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract].
Digman, M.F., Shinners, K.J., Dien, B.S., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass and corn stover [abstract]. Abstracts of the 29th Symposium on Biotechnology for Fuels and Chemicals, April 29-May 2, 2007, Denver, Colorado. p. 147.
Digman, M.F., Shinners, K.J., Dien, B.S., Hatfield, R.D., Li, X., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass. In: Proceedings of 2007 Annual Meeting of American Society of Agricultural and Biological Engineers, June 17, 2007, Minneapolis, Minnesota. Paper No. 071021.
Anderson, W.F., Dien, B.S., Peterson, J., Akin, D.E. 2007. Genotypic Evaluation of Bermudagrass for Conversion to Ethanol. Proceedings: 29th Symposium on Biotechnology for Fuels and Chemicals, Denver, CO, April 29-May 2, 2007.
Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. 233rd American Chemical Society National Meeting, March 25-29, 2007, Chicago, Illinois. Available: http://oasys2.confex.com/acs/233nm/techprogram/P1061547.htm.
Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. In: 38th Northwest Alfalfa Seed Growers Association Winter Seed Conference Proceedings, January 22-23, 2007, Las Vegas, Nevada. p. 17-19.
Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. In: Midwest Forage Association Symposium and Annual Meeting Proceedings, January 30-31, 2007, Wisconsin Dells, Wisconsin. CD-ROM.
Cotta, M.A., Dien, B.S., Iten, L.B., Jung, H.G., Lamb, J.F., Vogel, K.P., Mitchell, R., Sarath, G., Casler, M.D., Weimer, P.J. 2006. Forage energy crops as feedstocks for production of fuel ethanol [abstract]. Biocatalysis and Bioenergy. p. 21.
Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275.
Jordan, D.B., Dien, B.S., Li, X., Cotta, M.A. 2006. Hemicellulases for mediating biomass saccharification. In: Proceedings of Renewable Energy 2006, October 9-13, 2006, Makuhari Messe, Chiba, Japan. p. 1036-1041.
Dien, B.S., Saha, B.C. 2006. Review of current research directions for producing biological ethanol at the National Center for Agricultural Utilization Research [abstract]. Advancing Renewable Energy. p. 27.
Lopez, M.J., Vargas-Garcia, M., Suarez-Estrella, F., Nichols, N.N., Dien, B.S., Moreno, J. 2007. Lignocellulose-degrading enzymes produced by the ascomycete Coniochaeta ligniaria and related species: application for a lignocellulosic substrate treatment. Enzyme and Microbial Technology. 40:794-800.
Cotta, M.A., Dien, B.S., Li, X., Ladisch, M., Mosier, N., Tyner, W., Woodson, R., Blaschek, H., Dale, B., Shanks, B., Verkade, J. 2006. Pretreatment and hydrolysis of distiller's grains to fermentable sugars: an integrated approach by the Midwest Consortium for sustainable biobased products and energy [abstract]. Corn Utilization. Paper No. 24.
Nichols, N.N., Mertens, J.A., Dien, B.S. 2006. Identification and transcriptional profiling of furoic acid metabolism genes in Pseudomonas putida [abstract]. American Society for Microbiology. Paper No. Q-449.
Dien, B.S., Nichols, N.N., Li, X., Cotta, M.A. 2006. An overview of recent advancements in lignocellulose to ethanol conversion technology [abstract]. International Conference on Bioenergy. p. 8.
Ladisch, M., Tyner, W., Mosier, N., Cotta, M.A., Dien, B.S., Blaschek, H., Dale, B., Shanks, B., Petersen, G. 2006. Research challenges and opportunities for cellulose conversion technology in a dry mill pathway [abstract]. Biotechnology for Fuels and Chemicals. p. 48.
Li, X., Jordan, D.B., Ximenes, E.A., Cotta, M.A., Dien, B.S., Hughes, S.R. 2006. Codon optimization of an A+T rich xylanase gene from the anaerobic fungus Orpinomyces PC-2 for expression by Trichoderma reesei [abstract]. In: Proceedings of the 9th International Workshop on Trichoderma and Gliocladium, April 6-8, 2006, Vienna, Austria. Paper No. T3.
Slininger, P.J., Dien, B.S., Gorsich, S.W., Liu, Z. 2006. Nitrogen source and mineral optimization enhance D-xylose conversion to ethanol by the yeast Pichia stipitis NRRL Y-7124. Applied Microbiology and Biotechnology. 72(6):1285-1296.
Cotta, M.A., Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Weimer, P.J., Iten, L.B., Mitchell, R., Sarath, G. 2006. Development of forage crops as feedstocks for production of fuel ethanol [abstract]. International Conference on Bioenergy. p. I-5.
Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2006. Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass. Biomass and Bioenergy. 30:880-891.
Dien, B.S., Li, X., Iten, L.B., Jordan, D.B., Nichols, N.N., O Bryan, P.J., Cotta, M.A. 2006. Enzymatic saccharification of hot-water pretreated corn fiber for production of monosaccharides. Enzyme and Microbial Technology. 39:1137-1144.
Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Hughes, S.R., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using genetically engineered Escherichia coli FBR5: membrane and fixed cell reactors [extended abstract]. American Institute of Chemical Engineers. Paper No. 589g.
Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using Escherichia coli FBR5: membrane and fixed cell reactors [abstract]. American Institute of Chemical Engineers. Paper No. 589g.
Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.
Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation of sugars in corn stover dilute acid hydrolysates [abstract]. Society of Industrial Microbiology. p. 85.
Slininger, P.J., Dien, B.S., Gorsich, S.W., Liu, Z. 2005. Mineral and nitrogen source optimization enhance d-xylose conversion to ethanol by the yeast Pichia stipitis [abstract]. Society of Industrial Microbiology Annual Meeting. Paper No. P07.
Qureshi, N., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2006. Genetically engineered Escherichia coli for ethanol production from xylose: substrate and product inhibition and kinetic parameters. Institution of Chemical Engineers Transactions. 84(2):114-122.
Dien, B.S., Li, X., Jordan, D.B., Nichols, N.N., Iten, L.B., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. International Starch Technology. p. 90.
Nichols, N.N., Dien, B.S., Wu, V., Cotta, M.A. 2005. Use of field pea starch as a feedstock for ethanol fermentation [abstract]. International Starch Technology. p. 96.
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2006. Metabolic engineering of a Lactobacillus plantarum double LDH knockout strain for enhanced ethanol production. Journal of Industrial Microbiology and Biotechnology. 33(1):1-7.
Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A., Singh, V. 2005. Hydrolysis and fermentation of pericarp and endosperm fibers recovered from enzymatic corn dry-grind process. Cereal Chemistry. 82(5):616-620.
Dien, B.S., Li, X., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. Biotechnology for Fuels and Chemicals. Paper No. B-36.
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation by overexpressing a Gram-positive PDC gene in Lactobacillus plantarum strain TF103 [abstract]. Biotechnology for Fuels and Chemicals Symposium Proceedings, May 1-4, 2005, Denver, Colorado. p. 158.
Liu, S., Dien, B.S. 2005. Metabolic engineering of Lactobacillus brevis for ethanol production [abstract]. The World Congress on Industrial Biotechnology and Bioprocessing. p. 23.
Dien, B.S., Iten, L.B., Skory, C.D. 2005. Converting herbaceous energy crops to bioethanol: a review with emphasis on pretreatment processes. In: Hou, C.T., editor. Handbook of Industrial Biocatalysis. Chapter 23. Boca Raton, FL: Taylor & Francis Group. p. 1-11.
Mosier, N.S., Hendrickson, R., Brewer, M., Ho, N., Sedlak, M., Dreshel, R., Welch, G., Dien, B.S., Aden, A., Ladisch, M.R. 2005. Industrial scale-up of ph-controlled liquid hot water pretreatment of corn fiber for fuel ethanol production. Applied Biochemistry and Biotechnology. 125:77-97.
Weimer, P.J., Dien, B.S., Springer, T.L., Vogel, K.P. 2005. In vitro gas production as a surrogate measurement of the fermentability of cellulosic biomass. Applied Microbiology Biotechnology. 67:52-58.
Dien, B.S., Whitehead, T.R., Nichols, N.N., Skory, C.D., Cotta, M.A. 2004. Recombinant biocatalysts for converting sugar mixtures to lactic acid [abstract]. Great Lakes Regional American Chemical Society Symposium. Paper No. 40.
Nichols, N.N., Dien, B.S., Lopez, M.J. 2004. A biological approach to removing inhibitory compounds from biomass sugars to be used for fermentation [abstract]. Great Lakes Regional American Chemical Society Symposium. Paper No. 36.
Li, X., Jordan, D.B., Dien, B.S., Cotta, M.A., Kane, P.M. 2004. Advances on enzymatic saccharification of lignocellulosic biomass [abstract]. Great Lakes Regional American Chemical Society. Paper No. 04-035.
Qureshi, N., Brining, H.R., Iten, L.B., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2004. Adsorbed cell dynamic biofilm reactor for ethanol production from xylose and corn fiber hydrolysate [abstract]. Great Lakes Regional American Chemical Society Symposium. p. 179.
Gorsich, S.W., Dien, B.S., Nichols, N.N., Slininger, P.J., Liu, Z. 2004. The Saccharomyces cerevisiae pentose phosphate pathway gene, rpe1, functions in furfural tolerance during fermentation [abstract]. Proceedings of the 11th International Congress on Yeasts in Science and Biotechnology. Paper No. PM24.
Singh, V., Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A. 2004. A comparison between conversion of pericarp and endosperm fiber from corn into ethanol. Proceedings of ASAE Annual International Meeting. p. 1-15.
Tumbleson, M., Lemuz, C., Dien, B.S., Singh, V., Belyea, R., Rausch, K. 2004. A standardized laboratory procedure for ethanol yield measurement [abstract]. International Fuel Ethanol Workshop. Paper No. 61.
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2004. Expression analysis of pyruvate decarboxylase genes from Zymobacter palmae and Sarcina ventriculi in Gram-positive lactic acid bacteria [abstract]. American Society for Microbiology. Paper No. O-099.
Dien, B.S., Whitehead, T.R., Nichols, N.N., Cotta, M.A. 2004. Engineering klebsiella oxytoca for production of lactic acid from hexose and pentose [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 2-08.
Li, X., Dien, B.S., Cotta, M.A. 2004. Growth of and enzyme production by Trichoderma reesei on corn fiber fractions [abstract]. Biotechnology For Fuels And Chemicals. p. 25.
Qureshi, N., Dien, B.S., Nichols, N.N., Cotta, M.A. 2004. Genetically engineered Escherichia coli for ethanol production from pentose sugars: substrate and product inhibition and kinetic parameters [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 5-23.
Nichols, N.N., Dien, B.S., Guisado, G.M., Lopez, M.J. 2004. Use of the ascomycete coniochaeta ligniaria to remove inhibitors from biomass-derived sugars [abstract]. Biotechnology For Fuels And Chemicals Symposium. Paper No. 2-10.
Liu, Z., Slininger, P.J., Dien, B.S., Berhow, M.A., Kurtzman, C.P., Gorsich, S.W. 2004. Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for hmf conversion to 2,5-bis-hydroxymethylfuran. Journal of Industrial Microbiology and Biotechnology. 31:345-352. Available http://dx.doi.org/10.1007/s10295-004-0148-3.
Dien, B.S., Nichols, N.N., Cotta, M.A. 2003. Fermentation of hexose and pentose sugar mixtures to lactic acid by recombinant bacteria [abstract]. American Institute Of Chemical Engineers. Paper No. 161D.
Mosier, N.S., Kim, Y., Zeng, M., Hendrickson, R., Dien, B.S., Welsh, G., Ladisch, M. 2003. Optimization of controlled pH liquid hot water pretreatment of corn fiber and stover [abstract]. American Institute Of Chemical Engineers. Paper No. 163d.
Cotta, M.A., Nichols, N.N., Dien, B.S., Li, X. 2003. Development of microbial biocatalysts to produce fuels from agricultural biomass. Proceedings 32nd annual United States Japan Natural Resources Protein Panel. p. 387-393.
Li, X., Ljungdahl, L.G., Ximenes, E.A., Chen, H., Felix, C.R., Cotta, M.A., Dien, B.S. 2004. Properties of a recombinant beta-glucosidase from the polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysis. Journal of Applied Biochemistry and Biotechnology. 113:233-250.
Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2004. Fermentation of "quick fiber" produced from a modified corn milling process into ethanol and recovery of corn fiber oil. Journal of Applied Biochemistry and Biotechnology. 115:937-950.
Qureshi, N., Dien, B.S., Saha, B.C., Ezeji, T.C., Blaschek, H.P., Cotta, M.A. 2003. Separation of butanol from fermentation broth by pervaporation: mass flux and energy balance [abstract]. American Institute of Chemical Engineers. Paper No. 162B.
Dien, B.S., Iten, L.B., Nichols, N.N., Cotta, M.A. 2003. Conversion of lignocellulose to ethanol using a recombinant E. coli strain [abstract]. Society of Industrial Microbiology. p. 102.
Dien, B.S., Bothast, R.J., Nichols, N.N., Cotta, M.A. 2003. Increasing the ethanol yield from a bushel of corn; prospects for biomass conversion [abstract]. International Starch Technology. Paper No. 2.
DIEN, B.S., NICHOLS, N.N., LI, X., COTTA, M.A., BOTHAST, R.J. CONVERSION OF CORN FIBROUS MATERIAL INTO ETHANOL. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. ABSTRACT P. 68.
EBENER, J.M., QURESHI, N., EZEJI, T.C., BLASCHEK, H.P., DIEN, B.S., COTTA, M.A. CORN FIBER HYDROLYSIS AND FERMENTATION TO BUTANOL USING CLOSTRIDIUM BEIJERINCKII BA101. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. PAPER NO. 2-14.
Mosier, N., Hendrickson, R., Dreschel, R., Dien, B.S., Bothast, R.J., Welch, G., Ladisch, M.R. 2003. Principles and economics of pretreating cellulose in water for ethanol production [abstract]. American Chemical Society. Paper No. 103.
Dien, B.S., Nichols, N.N., Iten, L.B., Bothast, R.J. 2004. Enhancement of ethanol yield from the corn dry grind process by fermentation of the kernel fiber fraction. In: Nelson, W.M., editor. Agricultural Applications in Green Chemistry. Chap. 6. Washington, DC:American Chemical Society. p. 63-77.
DIEN, B.S., BOTHAST, R.J., NICHOLS, N.N., COTTA, M.A. THE U.S. CORN ETHANOL INDUSTRY: AN OVERVIEW OF CURRENT TECHNOLOGY AND FUTURE PROSPECTS. INTERNATIONAL SUGAR JOURNAL. 2002. V. 104. P. 204-211.