Technical Abstract: Lactobacillus intermedius B-3693 was selected as a good producer of mannitol from 9 strains (Lactobacillus brevis B-1836, L. buchneri B-1860, L. cellobiosus B-1840, L. fermentum B-1915, L. intermedius B-3693, Leuconostoc amelibiosum B-742, L. citrovorum B-1147, L. mesenteroides subsp. dextranicum B-1120, and L. paramesenteroides B-3471) that produced mannitol from fructose, after screening 72 bacterial strains. The bacterium produced mannitol, lactic acid, and acetic acid from fructose in pH-controlled batch fermentation. Typical yields of mannitol, lactic acid, and acetic acid from 250 g/L fructose were 0.70, 0.16, and 0.12 g, respectively per g of fructose. The fermentation time was greatly dependent on fructose concentration (from 20 h at 150 g/L to 136 h at 300 g/L) but the product yields were not dependent on fructose level. Fed-batch fermentation decreased the time of maximum mannitol production from fructose (300 g/L) from 136 h to 92 h. One-third of fructose could be replaced with glucose, maltose, galactose, mannose, raffinose, or starch with glucoamylase (simultaneous saccharification and fermentation, SSF) and two-thirds of fructose could be replaced with sucrose. L. intermedius B-3693 did not co-utilize lactose, cellobiose, glycerol, and xylose with fructose. It produced lactic acid (70.4 ± 0.6 g/L) and ethanol (36.2 ± 1.0 g/L) but no acetic acid from glucose (150 g/L). The bacterium produced 21.3 ± 0.6 g lactic acid, 10.5 ± 0.3 g acetic acid, and 4.7 ± 0.0 g ethanol per L of fermentation broth from dilute acid (15% solids, 0.5% H2SO4, 121 deg C, 1 h) pretreated enzyme (cellulase, beta-glucosidase) saccharified corn fiber hydrolyzate.