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Active Bacterial Core Surveillance

Methodology - Laboratory Characterization


Pathogen

Test(s)

Neisseria meningitidis

Identification and serogrouping by conventional microbiological methods. Confirmation of serogroup and species identification using real-time PCR

Multilocus sequence typing, pulsed field gel electrophoresis, and serosubtyping on samples of isolates as part of special projects

Antimicrobial susceptibility testing of periodic samples of isolates

Haemophilus influenzae

Identification and serotyping (a-f) by conventional microbiological methods

Confirmation of serotype and species using real-time PCR as part of special projects

group A streptococcus

T and emm typing of all isolates

Sof sequence typing of a subset of isolates

Antimicrobial susceptibility testing of a subset of isolates

group B streptococcus

Serotyping of isolates from select surveillance areas

Antimicrobial susceptibility testing of isolates from select surveillance areas

Streptococcus pneumoniae

Antimicrobial susceptibility testing of all isolates

Serotyping of all isolates

Progressing toward clonal typing of all isolates using MLST

Characterization of resistance mechanisms of selected strains based on phenotypic resistance patterns

Methicillin-resistant Staphylococcus aureus

Confirmatory identification of all isolates

Antimicrobial susceptibility testing, selected isolates

Pulsed field gel electrophoresis and analysis using PulseNet methodology, selected isolates

SCCmec cassette typing, selected isolates

Toxin testing for PVL and TSST-1, selected isolates
 
 
Page Last Modified: February 16, 2009
Content source: National Center for Immunization and Respiratory Diseases / Division of Bacterial Diseases
 
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