Ordering Information
Product Images
WB: HUVEC (lane 1) and VEGF treated HUVEC cell lysate (lane 2) were probed with anti-phospho-eNOS (Ser1177), 1:1000 dilution
Anti-phospho-eNOS/NOS III (Ser1177)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Serum | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 a ... see more »
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 a ... see more »
Entrez Gene Number:
Description:
Anti-phospho-eNOS/NOS III (Ser1177)
Specificity:
Recognizes phosphorylated eNOS.
Key Applications:
Western Blotting
Species Reactivity:
Human
Isotype:
IgG
Immunogen:
synthetic peptide corresponding to amino acids 1172-1183 of human eNOS.
Modifications:
Phosphorylation
Molecular Weight:
132 kDa
Control:
Brain tissue
Presentation:
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Storage Conditions:
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- NOS3
- cNOS
- ECNOS
- eNOS
- EC-NOS
- NOSIII
Quality Assurance:
routinely evaluated by immunoblot from VEGF treated HUVEC cell lysates
Antibody Category:
Neuroscience
Antibody Sub-Category:
Oxidative Stress
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
Variable
Format Code:
Serum
Trade Name:
Upstate (Millipore)
Purification Method:
UnPurified
Format:
Serum
Product Overview:
Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Antibody Type:
Polyclonal Antibody
Host:
Rabbit