Antibody Validation Table

Target Protein	Company	Recommended Dilution	Source	Weight	Cross Reactivity	Positive Control	Cross Reactivity Validated by Western Blotting (Canine Samples)
Akt	COP:Cell Signaling	1:1000	Rabbit	60kDa	H,M,R	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP: Protocol- Tumor Western Blot-Tumor tissue
pAKT	COP:Cell Signaling	1:1000	Rabbit	60kDa	H,M,R,C,Hm	K7M2 NIH 3T3	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor Western Blot-Tumor tissue
elF4E	COP:Cell Signaling	1:1000	Rabbit	25kDa	H,M,R,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
P-elF4E	COP:Cell Signaling	1:1000	Rabbit	25 kDa	H,M,R,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
Ezrin	COP:Sigma	1:1000	Mouse	80 kDa	H	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor Western Blot-Tumor tissue
p-Ezrin	COP:Cell Signaling	1:1000	Rabbit	80 kDa		K7M2 NIH 3T3	COP:Protocol COP:Western Blot-Normal tissue COP: Protocol-Tumor Western Blot-Tumor tissue
ERM	COP:Cell Signaling	1:1000	Rabbit	75,80 kDa	H,M,R,MK	K7M2 A431	COP:Protocol COP:Western Blot-Tumor tissue COP:Protocol-Tumor Western Blot-Tumor tissue
P-ERM	COP:Cell Signaling	1:1000	Rabbit	75, 80 kDa	H,M,R,Mk	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor Western Blot-Tumor tissue
P-4E-BP1	COP:Cell Signaling	1:1000	Rabbit	15-20 kDa	H,M,R,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
p44/42 Map Kinase	COP:Cell Signaling	1:1000	Rabbit	42,44 kDa	H,M,R,Hm,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
P-P44/42 Map Kinase	Cell Signaling	1:2000	Mouse	42,44 kDa	H,M,R,Hm,Z	K7M2	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
p70 S6 Kinase	COP:Cell Signaling	1:1000	Mouse	70,85 kDa	H,M,R,Mk	K7M2 NIH 3T3	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue
P-p70 S6 Kinase	COP:Cell Signaling	1:1000	Mouse	70,85 kDa	H,M,R,Mk	K7M2 NIH 3T3	COP:Protocol COP:Western Blot-Normal tissue COP:Protocol-Tumor COP:Western Blot-Tumor tissue

For any questions please contact Christina Mazcko at mazckoc@mail.nih.gov

Antibody Validation Table

Antibody Validation Table

Labels