| | Principal Investigators
| Sanford P. Markey, PH.D. |
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Dr.
Markey is chief of the Laboratory
of Neurotoxicology of the Intramural Research Program,
National Institute of Mental Health in Bethesda, Maryland.
Dr. Markey received his A.B. from Bowdoin College and his Ph.D. in chemistry
from the Massachusetts Institute of Technology. In a faculty position in
Pediatrics and Pharmacology at the University of Colorado Medical Center,
Dr. Markey developed and applied gas chromatographic-mass spectrometric-computer
methods to the diagnosis of inborn errors of metabolism. Dr. Markey joined NIMH in
1974 and developed a laboratory to investigate the metabolism of small molecules
important in brain biochemistry. Dr. Markey has been a member of the Medical
Advisory Board of the American Parkinson Disease Association, the Instrumentation
Advisory Panel of Analytical Chemistry, the Executive Board of the American Society
for Mass Spectrometry, and is currently a member of the board of directors of
U.S. Human Proteome Organization (USHUPO). |
| Research Interests |
At NIMH, Dr.
Markey devised the first successful sensitive methods to quantify circulating melatonin
in humans, enabling studies to determine the effect of light and developmental stage on
this pineal secretion. His group then investigated the parkinsonian-producing neurotoxin
MPTP, a by-product of abused drug synthesis. Dr. Markey described MPTP, and determined
how its metabolism to an active metabolite, MPP+, produces the parkinsonian affects.
MPTP has been a most useful tool for the study of parkinsonism in humans, enabling
many therapeutic strategies to be tested in animals. In studies with Dr. Heyes,
Dr. Markey probed the chemistry of quinolinic acid, an endogenous excitotoxic
amino acid metabolite of tryptophan. Sensitive quantitative measurement of
quinolinate and its kinetic disposition demonstrated the role of CNS-derived
immune-mediated events in HIV-infection and other infectious diseases.
Dr. Markey’s laboratory research has changed from studying the quantitative
actions of small neurotoxins to the study of macromolecular protein complexes in
sub-cellular structures, particularly those relevant to normal neuronal development.
His laboratory has devised new separation methods to facilitate the identification
of proteins in complex mixtures and methods to quantify the relative and absolute
amounts of specific proteins. Several related projects are directed toward describing
the normal sub-cellular complexes in yeast and mammalian cells. Bioinformatics software tools
are being created to more fully utilize shotgun proteomic data and to parse and compare sets
of data from different experiments.
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| Representative Selected Recent Publications: |
- Ayse Dosemeci, Anthony J. Makusky, Ewa Jankowska-Stephens, Xiaoyu Yang, Douglas J. Slotta, and Sanford P. Markey:
Composition of the Synaptic PSD-95 Complex
Molecular & Cellular Proteomics, 6,10, 1749-60, 2007. (View PDF)
- Fujigaki H, Saito K, Lin F, Fujigaki S, Takahashi K, Martin BM, Chen CY, Masuda J, Kowalak J, Takikawa O, Seishima M, Markey SP:
Nitration and inactivation of IDO by peroxynitrite.
Journal of Immunology, 176: 372-379, 2006.
- Laurent C, Levinson DF, Schwartz SA, Harrington PB, Markey SP, Caprioli RM, Levitt P:
Direct profiling of the cerebellum by matrix-assisted laser desorption/ionization time-of-flight mass pectrometry: a methodological study in postnatal and adult mouse.
Journal of Neuroscience Research, 81(5):613-621, 2005. (View PDF)
- Xu M, Geer LY, Bryant SH, Roth JS, Kowalak JA, Maynard DM, Markey SP:
Assessing data quality of peptide mass spectra obtained by quadrupole ion trap mass spectrometry.
Journal of Proteome Research, 4: 300-305, 2005. (View PDF)
- Masuda J, Maynard DM, Nishimura M, Ueda T, Kowalak JA, Markey SP:
Fully automated micro- and nanoscale one- or two-dimensional high-performance liquid chromatography system for liquid chromatography-mass spectrometry compatible with non-volatile salts for ion exchange chromatography.
Journal of Chromatography A, 1063: 57-69, 2005. (View PDF)
- Yang X, Dondeti V, Dezube R, Maynard DM, Geer LY, Epstein J, Chen X, Markey SP, Kowalak JA:
DBParser: web-based software for shotgun proteomic data analyses.
Journal of Proteome Research, 3: 1002-1008, 2004. (View PDF)
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