Assessment of chromosome loss, gain or amplification in a sample of genomic DNA by competitive hybridization of two genomes against a target cell.
Identifies chromosomal aberrations |
|
Translocations (balanced) |
No |
Translocations (unbalanced) |
Yes |
Copy number changes |
Yes |
Additions (>10MB) |
Yes |
Deletions (>10MB) |
Yes |
Deletions of specific genes/loci |
Sometimes |
Inversions |
No |
Insertions |
No |
Identifies presence of markers |
Sometimes |
Distinguishes between double minutes and homogeneously staining regions |
No |
Identifies chromosomal origins |
Sometimes |
Identifies specific p/q arms/bands |
Sometimes |
Requires specifically labeled probes |
Yes |
More than two probes at one time |
Yes |
Requires viable material |
No |
Requires metaphase spreads |
Yes |
Applicable to interphase nuclei |
No |
Applicable to DNA extracted from paraffin embedded tissue |
Yes |
Affected by normal tissue contamination |
Yes |
Identifies tumor heterogeneity |
No |
Applicable for studies in other species |
Yes |