Inami et al., 2001
- Shredding
- 100 g dry seeds blended for 2 min
- Shredded seeds mixed with 900 ml lactose broth
- Followed BAM for sample pre-enrichment, enrichment and confirmation
- Sprouting
- 100 g seeds soaked in 200 ml sterile water for 18-20 h at 25:C.
- Sprouted for 3 days at room temperature
- 100 g germinated sprouts mixed with 900 ml lactose broth and blended for 2 min
- Followed BAM for sample pre-enrichment, enrichment and confirmation