|
| Peptone | 10 g |
| Beef extract | 5 g |
| NaCl | 20 g |
| 1000X Dye stock solution* | 1 ml |
| Agar | 15 g |
| Distilled water | 900 ml |
Adjust to pH 7.6. Boil to dissolve agar. Cool to 48-55°C.
| Bromthymol blue | 4.0 g |
| Cresol red | 4.0 g |
| Ethanol, 95% | 100 ml |
For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes. Dissolve dyes in ethanol for 4% (w/v) stock solution. Using 1 ml of this solution per liter of mCPC agar gives 40 mg bromthymol blue and 40 mg cresol red per liter.
| Cellobiose | 10 g |
| Colistin | 400,000 units |
| Polymyxin B | 100,000 units |
| Distilled water | 100 ml |
Dissolve cellobiose in distilled water by heating gently. Cool. Add antibiotics.
Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, dark green to green-brown.
NOTE: This medium, like TCBS, is very inhibitory and does not require autoclaving. Medium may be stored 2 weeks at refrigeration temperatures.
| Storage: | limit: 2 wks | Temperature: 4°C |
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
