Microbiological Findings on Sprout Operations Following FDA Guidance

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Author: Dr. Jed W. Fahey, Johns Hopkins School of Medicine

**Table of Contents**
Microbiological Findings on Sprout Operations Following FDA Guidance Overview Disclosure Effect of Leachate from sprouting broccoli seeds, on the growth of E. coli Antibiotic Potency of Sulforaphane Introduction Introduction Introduction Introduction Introduction Hold-and-Release Testing Hold-and-Release Testing	Seed treatment and sprout Production Seed treatment and sprout Production Data Collection Data Collection Microbial testing Microbial testing Method used for hold-and- release testing Distribution of number of drums composited into each sample for pathogen testing. Presumptive positive samples grouped by composite number of drums represented by the water sample tested. Summary Acknowledgement