Project Number: 3625-21000-049-03
Project Type: Memorandum of Understanding

Start Date: Nov 10, 2006
End Date: Nov 09, 2011

Objective:
The objective is to physically map and sequence the barley gene space, with the near-term need being the identification of the remainder of approximately 50,000 genes, including the 5' and 3' regulatory regions, and the long-term goal being an ordered physical map linked to the genetic map to accelerate crop improvement.

Approach:
The barley genome will be sequenced by an incremental approach that relies on strategies utilized with other large genomes. In brief: a BAC-based high information content fingerprint (HICF) physical map will form the foundation. Genomic Survey Sequencing (GSS) will provide a resource for further large-scale marker development and enhance anchoring the physical and genetic maps. BAC end sequences (BES), accumulated during GSS, will serve as sequence tagged connectors (STC) for supporting and facilitating local contig extension. Starting from these, the consortium will focus its efforts to advance the physical maps of all chromosomes. Chromosomal regions harboring important traits and regions selected for the study of cross-Triticeae genome dynamics and grass genome colinearity will be emphasized.