National Heart, Lung, and Blood Institute Division of Intramural Research
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Fly Embryo RNAi Project
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Microscopies and Staining Techniques
Brightfield Images    Confocal Images    Two-Photon Images    3D Image
Nervous system wild type embryos were examined using bright field, confocal, and two-photon microscopies. Antibody staining specific for the central and peripheral nervous system was performed. DAB (diaminobenzidine) staining was used for visualization under a brightfield microscope. Flurescent dyes were used if specimens were observed using confocal or two-photon microscopies. Embryos were kept intact to obtain an overal image of the nervous sytem from the lateral and ventral views. They were dissected (fillet preparation) to reveal detailed structures of the nervous system.
Brightfield Images


Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 1097 KB)
Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 776 KB)
Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 1042 KB)
Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 1117 KB)
Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 1038 KB)
Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 1135 KB)
Fly embryo nervous system wild type image

Fillet Preparation
download image
(File size: 1038 KB)
Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 1036 KB)
Fly embryo nervous system wild type image

Fillet Preparation
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(File size: 805 KB)
Fly embryo nervous system wild type image

Lateral
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(File size: 1203 KB)
Fly embryo nervous system wild type image

Lateral
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(File size: 1095 KB)
Fly embryo nervous system wild type image

Ventral
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(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 535 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 535 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1094 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1094 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1094 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Confocal Images


Fly embryo nervous system wild type image

Fillet Preparation
download image
(File size: 1098 KB)
Fly embryo nervous system wild type image

Fillet Preparation
download image
(File size: 894 KB)
Fly embryo nervous system wild type image

Fillet Preparation
download image
(File size: 777 KB)
Fly embryo nervous system wild type image

Fillet Preparation
download image
(File size: 1094 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 538 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 538 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1097 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1096 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 1111 KB)
 
Two-Photon Images


Fly embryo nervous system wild type image

Lateral
download image
(File size: 537 KB)
Fly embryo nervous system wild type image

Lateral
download image
(File size: 537 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 537 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 538 KB)
Fly embryo nervous system wild type image

Ventral
download image
(File size: 537 KB)
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Fly Embryo RNAi Project (Version 2.10)