Projects

DEVELOPMENT AND USE OF PHYLOGENETIC SYSTEMS TO ENHANCE FOOD SAFETY AND FOOD SECURITY Appropriated (D)   Accession Number: 410277

MANAGEMENT AND GENETIC CHARACTERIZATION OF AGRICULTURAL AND BIOTECHNOLOGICAL MICROBIAL RESOURCES Appropriated (D)   Accession Number: 413230

GENETIC CHANGES IN THE CANADIAN FUSARIUM GRAMINEARUM POPULATION AND THEIR EFFECT ON PATHOGENICITY, TOXIN PRODUCTION, FUNGICIDE SENSITIVITY Reimbursable (R)   Accession Number: 411612

Publications

Spore development and trichothecene mutants of Fusarium graminearum - (Abstract)

Gale, L.R., Harrison, S.A., Milus, G.A., Ochocki, G.E., O Donnell, K., Ward, T.J., Kistler, H.C. 2007. Spore Development and Trichothecene Mutants of Fusarium graminearum [abstract]. Proceedings of the National Fusarium Head Blight Forum. p. 26.

An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminerarum in North America - (Peer Reviewed Journal)

Ward, T.J., Clear, R.M., Rooney, A.P., O Donnell, K., Gaba, D., Patrick, S., Starkey, D.E., Gilbert, J., Geiser, D.M., Nowicki, T.W. 2008. An adaptive evolutionary shift in Fusarium head blight pathogen populations is driving the rapid spread of more toxigenic Fusarium graminerarum in North America. Fungal Genetics and Biology. 45(4):473-484.

The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization - (Peer Reviewed Journal)

Cuomo, C.A., Guldener, U., Xu, J., Trail, F., Turgeon, B., Di Pietro, A., Walton, J.D., Ma, L., Baker, S.E., Rep, M., Adam, G., Antoniw, J., Baldwin, T., Calvo, S., Chang, Y., Decaprio, D., Gale, L., Gnerre, S., Goswami, R.S., Hammond-Kosack, K., Harris, L.J., Hilburn, K.L., Kennell, J., Kroken, S., Magnuson, J.K., Mannhaupt, G., Mauceli, E., Mewes, H., Mitterbauer, R., Muehlbauer, G., Munsterkotter, M., Nelson, D., O Donnell, K., Ouellet, T., Qi, W., Quesneville, H., Roncero, M.G., Seong, K., Tetko, I.V., Urban, M., Waalwijk, C., Ward, T.J., Yao, J., Birren, B.W., Kistler, H.C. 2007. The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization. Science. 317(5843):1400-1402.

Population subdivision of Fusarium graminearum sensu stricto in the Upper Midwestern United States - (Peer Reviewed Journal)

Gale, L.R., Ward, T.J., Balmas, V., Kistler, H.C. 2007. Population subdivision of Fusarium graminearum sensu stricto in the Upper Midwestern United States. Phytopathology. 97(11):1434-1439.

Phylogenetic diversity and microsphere array-based genotyping of human pathogenic Fusaria, including isolates from the multistate contact lens-associated U.S. keratitis outbreaks of 2005 and 2006 - (Peer Reviewed Journal)

O Donnell, K., Sarver, B., Brandt, M., Chang, D.C., Nobel-Wang, J., Park, B.J., Sutton, D., Benjamin, L., Lindsley, M., Padhye, A., Geiser, D.M., Ward, T.J. 2007. Phylogenetic diversity and microsphere array-based genotyping of human pathogenic Fusaria, including isolates from the multistate contact lens-associated U.S. keratitis outbreaks of 2005 and 2006. Journal of Clinical Microbiology. 45(7):2235-2248.

GLOBAL MOLECULAR SURVEILLANCE REVEALS NOVEL FUSARIUM HEAD BLIGHT SPECIES AND TRICHOTHECENE TOXIN DIVERSITY - (Peer Reviewed Journal)

Starkey, D.E., Ward, T.J., Aoki, T., Gale, L.R., Kistler, H.C., Geiser, D.M., Suga, H., Toth, B., Varga, J., O Donnell, K. 2007. Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversity. Fungal Genetics and Biology. 44(11):1191-1204.

A Single-Nucleotide-Polymorphism-BASED MULTILOCUS GENOTYPING ASSAY FOR SUBTYPING LINEAGE I ISOLATES OF LISTERIA MONOCYTOGENES - (Peer Reviewed Journal)

Ducey, T.F., Page, B., Usgaard, T.R., Borucki, M.K., Pupedis, K., Ward, T.J. 2007. A single-nucleotide-polymorphism-based multilocus genotyping assay for subtyping lineage I isolates of Listeria monocytogenes. Applied and Environmental Microbiology. 73(1):133-147.

THE PRESENCE OF GC-AG INTRONS IN N. CRASSA AND OTHER EUASCOMYCETES DETERMINED FROM ANALYSIS OF COMPLETE GENOMES: IMPLICATIONS FOR AUTOMATED GENE PREDICTION - (Peer Reviewed Journal)

Rep, M., Duyvesteijn, R.G., Gale, L., Usgaard, T.R., Cornelissen, B.J., Ma, L., Ward, T.J. 2006. The presence of GC-AG introns in N. crassa and other Euascomycetes determined from analysis of complete genomes: Implications for automated gene prediction. Genomics. 87:338-347.

TRICHOTHECENE AND ZEARALENONE PRODUCTION IN CULTURE, BY ISOLATES OF FUSARIUM PSEUDOGRAMINEARUM FROM WESTERN CANADA - (Peer Reviewed Journal)

Clear, R.M., Patrick, S.K., Gaba, D., Roscoe, M., Turkington, T.K., Demeke, T., Pouleur, S., Couture, L., Ward, T.J., O Donnell, K. 2006. Trichothecene and zearalenone production in culture, by isolates of Fusarium pseudograminearum from western Canada. Canadian Journal of Plant Pathology. 28:131-136.

CHROMOSOME COMPLEMENT OF THE FUNGAL PLANT PATHOGEN FUSARIUM GRAMINEARUM BASED ON GENETIC AND PHYSICAL MAPPING AND CYTOLOGICAL OBSERVATIONS - (Peer Reviewed Journal)

Gale, L.R., Bryant, J., Calvo, S., Giese, H., Katan, T., O Donnell, K., Suga, H., Taga, M., Usgaard, T.R., Ward, T.J., Kistler, H.C. 2005. Chromosome complement of the fungal plant pathogen Fusarium graminearum based on genetic and physical mapping and cytological observations. Genetics. 171:985-1001.

SUSPENSION MICROARRAY WITH DENDRIMER SIGNAL AMPLIFICATION ALLOWS DIRECT AND HIGH THROUGHPUT SUBTYPING OF LISTERIA MONOCYTOGENES FROM GENOMIC DNA - (Peer Reviewed Journal)

Borucki, M.K., Reynolds, J.O., Call, D., Ward, T.J., Page, B.T., Kadushin, J. 2005. Suspension Microarray with Dendrimer Signal Amplification Allows Direct and High-Throughput Subtyping of Listeria monocytogenes from Genomic DNA. Journal of Clinical Microbiology. 43(7):3255-3259.

FUSARIUM GRAMINEARUM IN THE U.S.: HETEROGENEOUS AND IN FLUX - (Abstract)

Gale, L.R., Bryant, J.D., Ochocki, G.E., Ward, T.J., Kistler, H.C. 2005. Fusarium graminearum in the U.S.: Heterogeneous and in flux [abstract]. Fungal Genetics Newsletter 52 (Suppl):63.

A GENETIC MAP OF GIBBERELLA ZEAE USING SEQUENCE-TAGGED SITES AND AFLPS. - (Abstract)

Gale, L.R., Bryant, J.D., Giese, H., Katan, T., O Donnell, K., Suga, H., Usgaard, T.R., Ward, T.J., Kistler, H.C. A genetic map of Gibberella zeae using sequence-tagged sites and aflps [abstract]. Fungal Genetics Newsletter 52 (Suppl):82.

EVOLUTION OF A LARGE RIBOSOMAL RNA MULTIGENE FAMILY IN FILAMENTOUS FUNGI: BIRTH AND DEATH OF A CONCERTED EVOLUTION PARADIGM - (Peer Reviewed Journal)

Rooney, A.P., Ward, T.J. 2005. Evolution of a large Ribosomal RNA multigene family in filamentous fungi: Birth and death of concerted evolution paradigm. Proceedings of the National Academy of Sciences. 102(14):5084-5089.

GENOMICS OF FUSARIUM GRAMINEARUM - (Abstract)

Kistler, H.C., Birren, B., Calvo, S., Cuomo, C., Gale, L.R., Gueldener, U., Ma, L., Muehlbauer, G., O Donnell, K., Trail, F., Ward, T.J., Xu, J. 2004. Genomics of fusarium graminearum. In: Proceedings of the 2nd International Symposium on Fusarium Head Blight, December 11-15, 2004, Orlando, FL, p.4.

THE USE OF HPLC-MS TO CHARACTERIZE TOXIN-PRODUCTION FROM FUSARIA BASED ON NEW PHYLOGENETIC CONCEPTS WITHIN THE GENUS. - (Abstract)

ZITOMER, N.C., GEISER, D.M., WARD, T.J., JIMINEZ-GASCO, M., ARCHIBALD, D.D., O DONNELL, K., KULDAU, G.A. THE USE OF HPLC-MS TO CHARACTERIZE TOXIN-PRODUCTION FROM FUSARIA BASED ON NEW PHYLOGENETIC CONCEPTS WITHIN THE GENUS.. AMERICAN PHYTOPATHOLOGICAL SOCIETY ABSTRACTS. 2004.

A GENETIC MAP OF FUSARIUM GRAMINEARUM USING SEQUENCED-TAGGED SITES AND AFLP MARKERS - (Abstract)

GALE, L.R., BRYANT, J.D., GIESE, H., KATAN, T., O DONNELL, K., SUGA, H., USGAARD, T.R., WARD, T.J., KISTLER, H.C. A GENETIC MAP OF FUSARIUM GRAMINEARUM USING SEQUENCED-TAGGED SITES AND AFLP MARKERS. AMERICAN PHYTOPATHOLOGICAL SOCIETY ABSTRACTS. 2004.

INTRASPECIFIC PHYLOGENY AND LINEAGE GROUP IDENTIFICATION BASED ON THE PRFA VIRULENCE GENE CLUSTER OF LISTERIA MONOCYTOGENES - (Peer Reviewed Journal)

Ward, T.J., Gorski, L.A., Borucki, M.K., Mandrell, R.E., Hutchins, J., Pupedis, K. 2004. Intraspecific phylogeny and lineage group identification based on the Prfa virulence gene cluster of Listeria monocytogenes. Journal of Bacteriology. 2004. 186(15):4994-5002.

GENEALOGICAL CONCORDANCE BETWEEN THE MATING TYPE LOCUS AND SEVEN OTHER NUCLEAR GENES SUPPORT FORMAL RECOGNITION OF NINE PHYLOGENETICALLY DISTINCT SPECIES WITHIN THE FUSARIUM GRAMINEARUM CLADE - (Peer Reviewed Journal)

O Donnell, K., Ward, T.J., Geiser, D.M., Kistler, H.C., Aoki, T. 2004. Genealogical concordance between the mating type locus and seven other nuclear genes support formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genetics and Biology. 41:600-623.

FUSARIUM-ID V.1.0: A DNA SEQUENCE DATABASE FOR IDENTIFYING FUSARIUM - (Peer Reviewed Journal)

Geiser, D.M., Delmar Jimenez-Gasco, M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T.J., Zhang, N., Kuldau, G.A., O Donnell, K. 2004. Fusarium-ID v.1.0: a DNA sequence database for identifying Fusarium. European Journal of Plant Pathology. 110(4):473-479.

GLOBAL GENETIC DIVERSITY OF FUSARIUM GRAMINEARUM CLADE SPECIES AND THEIR MYCOTOXIN POTENTIAL - (Abstract)

O Donnell, K., Ward, T.J., Geiser, D.M., Kistler, H.C., Gale, L.R., Aoki, T. 2003. Global genetic diversity of fusarium graminearum clade species and their mycotoxin potential [abstract]. National Fusarium Head Blight Forum Proceedings. p. 149.

DETECTION OF DISTINCT SUBPOPULATIONS OF FUSARIUM GRAMINEARUM, LINEAGE 7 - (Abstract)

Gale, L.R., Ward, T.J., Balmas, V., Kistler, H.C. 2003. Detection of distinct subpopulations of fusarium graminearum, lineage 7 [abstract]. National Fusarium Head Blight Forum Proceedings. p. 139.

WHOLE GENOME SEQUENCE OF FUSARIUM GRAMINEARUM, LINEAGE 7 - (Abstract)

Kistler, H.C., Birren, B., Calvo, S., Galagan, J., Gale, L.R., Ma, L., Muehlbauer, G., O Donnell, K., Trail, F., Ward, T.J., Xu, J. 2003. Whole genome sequence of fusarium graminearum, lineage 7 [abstract]. National Fusarium Head Blight Forum Proceedings. p. 141.

POPULATION SUBDIVISION IN FUSARIUM GRAMINEARUM LENEAGE 7 IN THE UNITED STATES IS CORRELATED WITH TOXIN CHEMOTYPE - (Abstract)

Gale, L.R., Ward, T.J., Balas, V., Kistler, H.C. 2003. Population subdivision in Fusarium graminearum lineage 7 in the United States is correlated with toxin chemotype [abstract]. Fungal Genetics Newsletter. 50:145.

ISOLATION AND CHARACTERIZATION OF THE TRI5-CLUSTER OF FUSARIUM GRAMINEARUM F15 REQUIRED FOR 3-O-ACETYLDEOXYNIVALENOL BIOSYNTHESIS - (Peer Reviewed Journal)

KIMURA, M., TOKAI, T., O DONNELL, K., WARD, T.J., FUJIMURA, M., HAMAMOTO, H., SHIBATA, T., YAMAGUCHI, I. ISOLATION AND CHARACTERIZATION OF THE TRI5-CLUSTER OF FUSARIUM GRAMINEARUM F15 REQUIRED FOR 3-O-ACETYLDEOXYNIVALENOL BIOSYNTHESIS. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2003. V. 539 (1-3). P. 105-110.

DISCORDANT EVOLUTION OF TRICHOTHECENE TOXINS AND SPECIES WITHIN THE FUSARIUM GRAMINEARUM SPECIES COMPLEX: PHYLOGENETIC EVIDENCE FROM MULTIGENE GENEALOGIES - (Abstract) - (30-Jan-03)

EVOLUTION OF THE FUSARIUM GRAMINEARUM SPECIES COMPLEX - (Abstract) - (17-Aug-02)

ANCIENT POLYMORPHISM AND ADAPTIVE EVOLUTION IN THE TRICHOTHECENE MYCOTOXIN GENE CLUSTER OF PHYTOPATHOGENIC FUSARIUM - (Abstract) - (03-Jul-02)