ARS | Publication request: A GENETIC MAP OF GIBBERELLA ZEAE USING SEQUENCE-TAGGED SITES AND AFLPS.

Submitted to: Fungal Genetics Conference/Asilomar
Publication Type: Abstract
Publication Acceptance Date: March 20, 2005
Publication Date: March 20, 2005
Citation: Gale, L.R., Bryant, J.D., Giese, H., Katan, T., O Donnell, K., Suga, H., Usgaard, T.R., Ward, T.J., Kistler, H.C. A genetic map of Gibberella zeae using sequence-tagged sites and aflps [abstract]. Fungal Genetics Newsletter 52 (Suppl):82.

Technical Abstract: A genetic map of Gibberella zeae (anamorph Fusarium graminearum) was constructed using a cross between nitrate-nonutilizing (nit) mutants of strain PH-1 and a Minnesota field strain, 00-676. A total of 111 ascospore progeny were analyzed for segregation at 237 loci. Genetic markers consisted of SNPs (detected as dCAPs, n=86 or CAPs, n=47), AFLPs (n=71), VNTRs (n=27), and six others. While 213 markers exhibited Mendelian inheritance, segregation distortion was noted for 17 markers at four genomic locations. A linkage map was generated using JoinMap 3.0 and a LOD threshold value of 4.0. Eleven linkage groups were obtained, covering 1154 cM and anchoring 99.8% of the sequence assembly. All linkage groups and anchored supercontigs were assembled into four chromosomes, leaving only 11 smaller supercontigs (76,055 bp total) of the nuclear DNA not anchored. Comparison between physical and genetic distance along chromosomes revealed genomic regions with reduced recombination and recombinational hotspots. More information can be found at http://www.broad.mit.edu/annotation/fungi/fusarium. lianeg@umn.edu