Human
Genome News Archive Edition |
|
Sponsored
by the U.S. Department of
Energy Human Genome Program
|
In this issue... 1997 Santa Fe Highlights Human Genome Project Administration In the News Publications Software and the Internet Funding Meeting Calendars & Acronyms |
JGI Clone Resource TaskLarge-insert clones for sequencing are being mapped, selected, and validated at JGI member laboratories. Jan-Fang Cheng (LBNL) discussed JGI development of clone-based maps for production sequencing; other team leaders are Norman Doggett (LANL) and Anne Olsen (LLNL). Among the major goals are the complete closure and validation of existing maps of chromosomes 5, 16, and 19; selection of new mapping targets; and creation of pools and high-density filters of newly approved BAC libraries for STS screening. Cheng observed that this year's challenge is to build templates for the FY 1999 ramp-up and beyond. Optimistic goals are to generate 70 Mb of contigs longer than 1 Mb, with associated restriction mapping data and over tenfold genome coverage. Summarizing the current map status of chromosomes 16, 19, and 5, Cheng reported that more than 70 Mb of contigs greater than 300 kb has been generated, with over 25 contigs (40 Mb) larger than 1 Mb. Cheng pointed out that before JGI was established, the three laboratories used different clone types as well as different mapping approaches. Currently, the mapping plan calls for three autonomous map-production teams, although ideally one major production site will generate templates for PSF; a centralized clone repository will be set up with 1-Mb contigs prioritized for sequencing. Breakdown for the clone resource task is resource production (15%), pilot R&D projects (15%), STS-content mapping (35%), and restriction mapping (35%). Cheng observed that, unlike sequencing strategies, mapping techniques evolve quickly and scientists want to retain flexibility. The electronic form of the newsletter may be cited in the following
style: |
Send the url of this page to a friend
Last modified: Wednesday, October 29, 2003
Home * Contacts * Disclaimer
Base URL: www.ornl.gov/hgmis
Site sponsored by the U.S. Department of Energy Office of Science, Office of Biological and Environmental Research, Human Genome Program