Mapping with STCs and STSs
STCs
An STC is a short stretch of sequence read from one end of the human DNA insert in a clone. BAC clone STCs can be useful in a number of ways. First, STCs help researchers expand contigs, as outlined in the article. Second, when the insert length is determined, the STC spacing helps verify the contiguous sequence created by assembly software. Third, BACs with STCs serve to physically define and thus "capture" gaps that occur when sequencing biochemistry is stalled by occasional difficult-to-read stretches of DNA sequence. Finally, STC reads can be used for the design of STSs.
STSs
An STS is a DNA segment that can be copied repeatedly by PCR without amplifying unwanted DNA regions from the source genome. STS markers have been used by members of the International RH Mapping Consortium to construct the RH maps that complement contig building. STSs generated from BAC STC reads are helping to enrich RH maps. Conversely, a mapped STS can be used to isolate a BAC (or any DNA clone type) from a library of clones representing a genome.
Return to BAC End Sequencing Speeds Large and Small Projects
The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v10n1-2).
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