|
Tris | 121.14 g |
NaCl | 117 g |
Concentrated HCl: as needed for pH 7.0 | |
Distilled water to make 1 liter |
Dissolve in several hundred ml distilled water. Adjust pH to 7.0 with concentrated HCl. Bring volume to 1 liter.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.