Research Abstracts from the
DOE Genome Contractor-Grantee Workshop IX

January 27-31, 2002 Oakland, CA

 

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Microbial Cell Project
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Low Dose Ionizing Radiation
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Sequencing Resources Abstract Index


7. Construction of BAC Libraries Using Sheared DNA

Kazutoyo Osoegawa, Chung Li Shu, and Pieter J. de Jong


8. BAC Library End Sequencing in Support of Whole Genome Assemblies

David C. Bruce, Mark O. Mundt, Kim K. McMurry, Linda J. Meincke, Donna L. Robinson, Norman A. Doggett, and Larry L. Deaven


9. An Approach to Filling Gaps in the Sequence of the Human Genome

X.-N. Chen, P. Bhattacharyya, S. Y. Zhao, M. Sekhon, J. McPherson, M. Wang, U.-J. Kim, H. Shizuya, M. Simon, and J. R. Korenberg


10. Isolation of Segments Missing from the Draft Human Genome Sequence Using Yeast

N. Kouprina, G. Solomon, S.-H. Leem, A. Ly, E. Pak, J. C. Barrett, and V. Larionov


11. Recent Segmental Duplications: A Dynamic Source of Gene Innovation and Complex Regions of Sequence Assembly

J. A. Bailey, J. E. Horvath, M. E. Johnson, M. Rocchi, and E. E. Eichler


12. Pooling DNA Clones for Shotgun Sequencing

Richard Gibbs and the staff of the Baylor College of Medicine-Human Genome Sequencing Center, Wei Wen Cai, and Allan Bradley


13. Production Clone Rearraying Using the QBot (Genetix Ltd.) and the LANL Cherrypicking Program

John J. Fawcett, James Colehan, Lyn Honeyborne, Bill Stevenson, David C. Bruce, Norman A. Doggett, and Larry L. Deaven


14. Applications of Isothermal Rolling Circle Amplification in a High-Throughput Sequencing Environment

John C. Detter, Jamie M. Jett, Andre R. Arellano, Alicia R. Ferguson, Kristie Tacey, Mei Wang, Heidi C. Turner, Susan M. Lucas, Ken Frankel, Paul Predki, Dan Rokhsar, Paul M. Richardson, and Trevor L. Hawkins


15. Efficient Isothermal Amplification of Single DNA Molecules

Stanley Tabor and Charles Richardson


16. Amplification of BAC DNA with Rolling Circular Amplification

Cliff S. Han, Judy Tesmer, Linda L. Meincke, Donna L. Robinson, Connie S. Campbell, Larry L. Deaven, and Norman A. Doggett


17. A Single-Copy, Amplifiable Plasmid Vector That Uses Homing Endonuclease   Recognition Sites to Facilitate Bidirectional Nested Deletion Sequencing of Difficult Regions

John J. Dunn, Laura Praissman, Laura-Li Butler-Loffredo, and Sean McCorkle


18. DENS: Finishing Without Custom Primers

Levy Ulanovsky, Olga Chertkov, Malinda Stalvey, Marie-Claude Krawczyk, David Hill, David Bruce, Mark Mundt, Larry Deaven, and Norman Doggett


19. High Throughput Synthesis of Oligonucleotides in Support of Finishing

L. Sue Thompson, Mark Mundt, David Bruce, Larry Deaven, and Norman Doggett


20. Automated 384-Well Purification for Terminator Sequencing Products

Chris Elkin, Hitesh Kapur, David Humphries, Troy Smith, and Trevor Hawkins


21. Whole Genome Direct Sequencing: Completion of Microbial Genome and Mammalian BAC Projects using ThermoFidelase, Fimer and D-Strap Technologies

S. Kozyavkin, A. Malykh, K. Mezhevaya, A. Morocho, N. Polouchine, V. Shakhova, O. Shcherbinina, and A. Slesarev


22. A Tape Conveyer System for Storage and Distribution of Biological Samples

Ger van den Engh and Juno Choe


23. Developing a High Throughput Lox Based Recombinatorial Cloning System

Robert Siegel, Raj Jain, Nileena Velappan, Leslie Chasteen, and Andrew Bradbury


24. Plant Mini-Chromosome Vectors

J. Mach and H. Zieler


25. Sampling Diversity with Mitochondrial Genomics

Jeffrey L. Boore, Nikoletta Danos, David DeGusta, H. Matthew Fourcade,  Lisa Gershwin, Allen Haim, Kevin Helfenbein, Martin Jaekel, Kirsten Lindstrom, J. Robert Macey, Susan Masta, Mónica Medina, Rachel Mueller, Marco Passamonti, Corrie Saux, Renfu Shao, and Yvonne Vallès


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