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U.S. Department of Health and Human Services
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Bacteriological Analytical Manual Online
December 2005

M193

Dey-Engley Broth



Tryptone 5.0 g
Yeast extract 2.5 g
Glucose 10.0 g
Sodium thioglycollate 1.0 g
Sodium thiosulfate 6.0 g
Sodium bisulfite 2.5 g
Polysorbate 80 5.0 g
Lecithin (soy bean) 7.0 g
Brom cresol purple 0.02 g
Distilled water 1 L

Autoclave 15 min. at 121°C.
Final pH 7.6 ± 0.2.


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