|
| Peptone | 20 g |
| NaCl | 30 g |
| 100X Dye stock solution* | 10 ml |
| Agar | 25 g |
| Distilled water | 900 ml |
Adjust to pH 8.2. Boil to dissolve agar. Autoclave for 15 min at 121°C. Temper to 50°C.
| Bromthymol blue | 0.6 g |
| 70% Ethanol | 100 ml |
For consistent medium color, use dye solution rather than repeatedly weighing out dry dyes.
| Cellobiose | 10 g |
| Distilled water | 100 ml |
Dissolve cellobiose in distilled water by heating gently. Cool and filter sterilize.
Add Solution 2 to cooled Solution 1, mix, and dispense into petri dishes. Final color, light blue.
Medium may be stored 2 weeks at refrigeration temperatures.
| Storage: | limit: 2 wks | Temperature: 4°C |
