The Combinatorial Methods project area in the
Biomaterials Group has generated results that characterize transitions
in cell response to scaffold physical and chemical parameters.
The biomaterials work has been done on 2D surfaces, whereas
most real tissue scaffolds are 3D.
This activity will employ 3D tissue scaffolds composed of polymer
blends who’s composition is taken from regions of 2D gradient
samples where cell behavior is distinctly different. We will
test for a confirmation of the 2D results in the 3D samples.
Experimental Approach
Osteoblasts
and macrophages respond to changes in composition of DTO / DTE
blends as shown to the right.
We
have obtained 3D salt-leached scaffolds composed of DTO / DTE
blends at several different compositions. These will be seeded
with MC-3T3 osteoblasts under static and flow culture conditions.
Cell
function will be determined by fluorescence microscopy / vinculin
staining and by quantitative RT-PCR testing for interlukin-1B
(immune response), and fibronectin (cell attachment).
Results
We
have obtained 3D salt-leached scaffolds composed of DTO /
DTE blends at several different compositions. These will be
seeded with MC-3T3 osteoblasts under static and flow culture
conditions.
Cell function will be determined by fluorescence microscopy
/ vinculin staining and by quantitative RT-PCR testing for
interlukin-1B (immune response), and fibronectin (cell attachment).
Structure
Cell Area (um2)
Focal Adhesions
Glass Coverslips
3777 ± 429
45 ± 4
PCL Surfaces
2501 ± 310
25 ± 2
PCL Scaffolds
1357 ± 158
11 ± 1
Future Activities
Extend 2D -->3D correlations to other cell responses
Contributors
Marcus Cicerone*
Matt Becker
Jeannie Stephens
Tithi Dutta-Roy
Francis Wang
Biomaterials Group
Polymers Division
Materials Science and Engineering Laboratory
We
have obtained 3D salt-leached scaffolds composed of DTO / DTE
blends at several different compositions. These will be seeded
with MC-3T3 osteoblasts under static and flow culture conditions.
