Table 1 version 5. Submission of Enteric Disease-associated Bacterial Isolates and Specimens
Table 1 version 5 PDF Cdc-pdf
Specimen | Service | Priority | Number to send, Submission scheme | Laboratory Examination Requested (test code, if applicable) | Contact Person§ | Frequency/timing | Accompanying Document |
---|---|---|---|---|---|---|---|
Clinical, food, environmental specimens for botulism investigations | Outbreak investigation | ASAP^ | All collected specimens | Botulism laboratory confirmation (CDC-10132) | Carolina Luquez or Janet Dykes | As soon as specimens collected | CDC form 50.34 |
Fecal, environmental and food specimens for Bacillus cereus, Clostridium perfringens and Staphylococcus aureus foodborne investigation | Outbreak investigation | ASAP^ | consult contact person for number to send | Detection – foodborne outbreak (CDC-10104, B. cereus ), (CDC-10111, C. perfringens ) or (CDC- 10113, S. aureus ) | Carolina Luquez or Gerry Gomez | as needed | CDC form 50.34 |
Clinical, food, and environmental specimens for the isolation of Campylobacter, Cronobacter, Listeria, Salmonella, Shigella , STEC and other diarrheagenic E. coli ^^, Vibrio, Yersinia (non-pestis) and other pathogenic Enterbacteriaceae | Outbreak/case investigation | ASAP^ | consult contact person for number to send | Enteric Isolation (CDC-10106) | Haley Martin or Nancy Strockbine | as needed | CDC form 50.34 |
Serum specimens: from suspect cases of Shiga-toxin producing E. coli , Campylobacter, Vibrio cholerae and Salmonella serotype Typhi | Outbreak investigation/surveillance |
As scheduled | consult contact person for type of specimen to send | Enteric serology: CDC- 10452 (E. coli); CDC- 10455 (Campylobacter); CDC-10454 (V. cholerae ); or CDC- 10453 (Salmonella serotype Typhi) | Rachael Aubert or Patti Fields | consult contact person for frequency/timing | CDC form 50.34 |
STEC (Shiga toxin-producing E. coli) broths | Reference/surveillance | ASAP^ | Any broths positive by Shiga toxin testing but culture-negative for STEC but which produce growth on subculture | Toxin typing, STEC isolation from enrichment broth (10105) | Nancy Strockbine or Devon Stripling or Haley Martin | as needed | CDC form 50.34 |
Organism | Service | Priority | Number to send, Submission scheme | Laboratory Examination Requested (test code, if applicable) | Contact Person§ | Frequency/timing | Accompanying Document |
---|---|---|---|---|---|---|---|
Aeromonas and related organisms | Reference/surveillance | At submitter’s convenience | any isolates you would like to have analyzed | Identification, subtyping (CDC-10120) | Cheryl Tarr or Monica Santovenia | as needed | CDC form 50.34 |
Bacillus cereus | Outbreak investigation | ASAP^ | consult contact person for number to send | Detection – foodborne outbreak (CDC-10104) | Carolina Luquez or Gerry Gomez | as needed | CDC form 50.34 |
Campylobacter | Outbreak investigation | ASAP^ | consult contact person for number to send | Campylobacter subtyping (CDC-10127) | Janet Pruckler or Rachael Aubert | every suspected outbreak | CDC form 50.34 |
Campylobacter (FoodNet Sites Only) | NARMSC | As scheduled | Every 2nd, 3rd, 5th, or all isolates (varies by state) | susceptibility testing for NARMS surveillance | Hayat Caidi or Jason Folster | at least quarterly | NARMS logsheet |
Campylobacter, Helicobacter, and related organisms | Reference/surveillance | At submitter’s convenience | isolates that cannot be identified or atypical / rare isolates | Identification (CDC-10126) | Janet Pruckler or Rachael Aubert | as needed | CDC form 50.34 |
Clostridium botulinum and other botulinum producing species of Clostridia | Reference/surveillance | ASAP^ | consult contact person for number to send | Botulinum toxin productin Clostridia subtyping (CDC-10133) |
Janet Dykes or Carolina Luquez | as needed | CDC form 50.34. DSAT approved Form 2 transfer |
Clostridium perfringens | Outbreak investigation | ASAP^ | consult contact person for number to send | Detection – foodborne outbreak (CDC-10111) | Carolina Luquez or Gerry Gomez | As needed | CDC form 50.34 |
Cronobacter sakazakii | Outbreak investigation | ASAP^ | Every isolate | CDC-10123 | Cheryl Tarr | Every suspected case | CDC form 50.34 |
Cronobacter species | Reference/surveillance | At submitter’s convenience, or ASAP^ if C.sakazakii has not been ruled out | Any isolates requiring speciation | CDC-10123 | Cheryl Tarr | As needed | CDC form 50.34 |
Enterobacteriaceae , other | Reference/surveillance | At submitter’s convenience | any isolates implicated in enteric disease | Identification, serotyping, subtyping (CDC-10123) | Cheryl Tarr | As needed | CDC form 50.34 |
Escherichia coli, diarrheagenic^^ | Outbreak investigation | ASAP^ | 3 representative isolates | Susceptibility testing (CDC-10116); other testing upon request | Nancy Strockbine or Devon Stripling or Haley Martin | In every suspected outbreak except multistate outbreak, in which case CDC will request | CDC form 50.34 |
Escherichia coli O157 | NARMSC | As scheduled | every 20th** | Susceptibility testing for NARMS surveillance | Hayat Caidi or Jason Folster | at least quarterly | NARMS logsheet |
Escherichia | Reference/surveillance | At submitter’s convenience | atypical isolates, rare serovars, or isolates that cannot be identified | Identification, serotyping, subtyping, virulence profiling (CDC-10114) | Nancy Strockbine or Devon Stripling or Haley Martin | As needed | CDC form 50.34 |
Listeria monocytogenes | Outbreak investigation | ASAP^* | every isolate from patients and implicated foods | Identification, serotyping, subtyping (CDC-10129) | Cheryl Tarr or Zuzana Kucerova | every time it is isolated/as soon as it is isolated | CDC form 50.34 |
Listeria, species other than monocytogenes | Reference/surveillance | ASAP^ | any isolates obtained from a normally sterile body site | Identification, serotyping, subtyping (CDC-10129) | Cheryl Tarr or Zuzana Kucerova | every time it is isolated/as soon as it is isolated | CDC form 50.34 |
Salmonella | Reference/surveillance | At submitter’s convenience | atypical isolates, rare serovars, or isolates that cannot be identified |
Salmonella identification and serotyping (CDC-10110) | Susan Van Duyne or Ana Lauer | as needed | CDC form 50.34 |
Salmonella (all non-typhoidal serotypes and serotype Paratyphi B) | NARMSC | As scheduled | every 20th** | susceptibility testing for NARMS surveillance | Hayat Caidi or Jason Folster | at least quarterly | NARMS logsheet |
Salmonella (all non-Typhi serotypes) | Outbreak investigation | ASAP^ | 3 representative isolates | Salmonella subtyping (CDC-10108) | Susan Van Duyne or Ana Lauer | Please send in every suspected outbreak unless multistate, in which case CDC will request | CDC form 50.34 |
Salmonella (typhoidal serotypes Paratyphi A, Paratyphi C and Typhi) | NARMSC | As scheduled | ALL | susceptibility testing for NARMS surveillance | Hayat Caidi or Jason Folster | at least quarterly | NARMS logsheet |
Salmonella serotype Typhi | Outbreak investigation | ASAP^ | 3 representative isolates |
Salmonella subtyping (CDC-10108) | Susan Van Duyne or Ana Lauer | In every suspected outbreak except multistate outbreak, in which case CDC will request | CDC form 50.34 |
Shigella | Reference/surveillance | At submitter’s convenience | any isolates you would like to have analyzed | Identification, subtyping (CDC-10114) | Nancy Strockbine or Devon Stripling or Haley Martin | as needed | CDC form 50.34 |
Shigella | Outbreak investigation | ASAP^ | 3 representative isolates |
Susceptibility testing (CDC-10116) | Nancy Strockbine or Devon Stripling or Haley Martin | In every suspected outbreak except multistate outbreak, in which case CDC will request | CDC form 50.34 |
Shigella | NARMSC | As scheduled | every 20th** | susceptibility testing for NARMS surveillance | Hayat Caidi or Jason Folster | at least quarterly | NARMS logsheet |
Staphylococcus aureus | Outbreak investigation | ASAP^ | consult contact person for number to send | Detection – foodborne outbreak (CDC-10113) | Carolina Luquez or Gerry Gomez | as needed | CDC form 50.34 |
Vibrio cholerae | Reference/surveillance | ASAP^ | every isolate | Identification, virulence testing (10119) | Cheryl Tarr or Monica Santovenia | every time it is isolated/as soon as it is isolated | CDC form 50.34 |
Vibrio mimicus | Reference/surveillance | At submitter’s convenience | every isolate | Identification, virulence testing (CDC-10120) | Cheryl Tarr or Monica Santovenia | at least quarterly | CDC form 50.34 |
Vibrio, non-cholerae | Outbreak investigation | ASAP^ | All isolates | Identification, subtyping (CDC-10122) | Cheryl Tarr or Monica Santovenia | every suspected outbreak | CDC form 50.34 |
Vibrio, non-cholerae | NARMSC | As scheduled | ALL | susceptibility testing for NARMS surveillance | Hayat Caidi or Jason Folster | at least quarterly | NARMS logsheet |
Vibrio and related organisms | Reference/surveillance | At submitter’s convenience | any isolates requiring speciation or that you would like to have analyzed | Identification, subtyping (CDC-10120) | Cheryl Tarr or Monica Santovenia | as needed | CDC form 50.34 |
Yersinia (non-pestis ) | Reference/surveillance | At submitter’s convenience | any isolates you would like to have analyzed | Identification, serotyping, biotyping, subtyping (CDC-10123) |
Cheryl Tarr | as needed | CDC form 50.34 |
^Isolates/specimens with a priority of “ASAP” should be shipped as soon as possible (within one business day of presumptive identification)
^^Escherichia coli , diarrheagenic = Shiga toxin-producing E. coli (STEC), Enterotoxigenic E. coli (ETEC), Enteropathogenic E. coli (EPEC), Enteroinvasive E. coli (EIEC) and Enteroaggregative E. coli ( EAEC)
CNational Antimicrobial Resistance Monitoring System
*Laboratories that are performing whole genome sequencing and submitting data in real time for Listeria can ship isolates quarterly instead of ASAP
**For non-typhoidal Salmonella, Shigella , and E. coli O157 isolates included in NARMS surveillance, the 1st isolate collected each year should be submitted, followed by every 20th isolate
§email addresses and phone numbers for contact persons are shown below:
General inquiries: entericbacteria@cdc.gov, 404-639-3334
Rachael Aubert: vrl7@cdc.gov, 404-639-3816
Hayat Caidi: foi0@cdc.gov, 404-639-0766
Janet Dykes: jkd1@cdc.gov, 404-639-3625
Patti Fields: pif1@cdc.gov, 404-639-1748
Jason Folster: gux8@cdc.gov, 404-639-4948
Gerry Gomez: goe4@cdc.gov, 404-639-0537
Zuzana Kucerova: zik0@cdc.gov, 404-718-4143
Ana Lauer: ybp6@cdc.gov, 404-639-2117
Carolina Luquez: fry6@cdc.gov, 404-639-0896
Haley Martin: hvw0@cdc.gov, 404-639-1612
Janet Pruckler: jmp5@cdc.gov, 404-639-4770
Monica Santovenia: ixi9@cdc.gov, 404-718-1446
Devon Stripling: euo4@cdc.gov, 404-639-2251
Nancy Strockbine: nas6@cdc.gov, 404-639-4186
Cheryl Tarr: crt6@cdc.gov, 404-639-2011
Susan Van Duyne: mdv9@cdc.gov, 404-639-0186
Public health officials (at the state and territorial public health laboratories and at CDC) can communicate about pending and current isolate requests via email
(entericbacteria@cdc.gov), a phone call to the Enteric Diseases Laboratory Branch at 404-639-3334, or by contacting specific personnel listed in Table 1 version 5.