Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primers and Probes
NOT FOR VIRAL TESTING USE
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These sequences are intended to be used for the purposes of respiratory virus surveillance and research. The recipient agrees to use them in compliance with all applicable laws and regulations. Every effort has been made to assure the accuracy of the sequences, but CDC cannot provide any warranty regarding their accuracy. The recipient can acknowledge the source of sequences in any oral presentations or written publications concerning the research project by referring to the Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
| Name | Description | Oligonucleotide Sequence (5’>3’) | Label1 | Final Conc. |
|---|---|---|---|---|
| 2019-nCoV_N1-F | 2019-nCoV_N1 Forward Primer | GAC CCC AAA ATC AGC GAA AT | None | 500nM |
| 2019-nCoV_N1-R | 2019-nCoV_N1 Reverse Primer | TCT GGT TAC TGC CAG TTG AAT CTG | None | 500nM |
| 2019-nCoV_N1-P | 2019-nCoV_N1 Probe | FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1 | FAM, BHQ-1 | 125nM |
| 2019-nCoV_N1-P | 2019-nCoV_N1 Probe | FAM-ACC CCG CAT /ZEN/ TAC GTT TGG TGG ACC-3IABkFQ | FAM, ZEN, 3IABkFQ | 125nM |
| 2019-nCoV_N2-F | 2019-nCoV_N2 Forward Primer | TTA CAA ACA TTG GCC GCA AA | None | 500nM |
| 2019-nCoV_N2-R | 2019-nCoV_N2 Reverse Primer | GCG CGA CAT TCC GAA GAA | None | 500nM |
| 2019-nCoV_N2-P | 2019-nCoV_N2 Probe | FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1 | FAM, BHQ-1 | 125nM |
| 2019-nCoV_N2-P | 2019-nCoV_N2 Probe | FAM-ACA ATT TGC /ZEN/ CCC CAG CGC TTC AG-3IABkF | FAM, ZEN, 3IABkFQ | 125nM |
| RP-F | RNAse P Forward Primer | AGA TTT GGA CCT GCG AGC G | None | 500nM |
| RP-R | RNAse P Reverse Primer | GAG CGG CTG TCT CCA CAA GT | None | 500nM |
| RP-P | RNAse P Probe |
FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1 | FAM, BHQ-1 | 125nM |
| RP-P | RNAse P Probe |
FAM-TTC TGA CCT /ZEN/ GAA GGC TCT GCG CG-3IABkFQ | FAM, ZEN, 3IABkFQ | 125nM |
1TaqMan® probes are labeled at the 5′-end with the reporter molecule 6-carboxyfluorescein (FAM) and with the quencher, Black Hole Quencher 1 (BHQ-1) (Biosearch Technologies, Inc., Novato, CA) at the 3′-end. TaqMan® probes can also be labeled at the 5′-end with the reporter molecule 6- carboxyfluorescein (FAM) and with a double quencher, ZEN™ Internal Quencher positioned between the ninth (9th) and tenth (10th) nucleotide base in the oligonucleotide sequence and Iowa Black® FQ (3IABkFQ) located at the 3’-end (Integrated DNA Technologies, Coralville, IA).
Note: Oligonucleotide sequences are subject to future changes as the 2019-Novel Coronavirus evolves.