United States
                       Environmental Protection
                       Agency
                               Department of the Army
                               US Army Corps of Engineers
EPA 823-F-94-002
June 1994
                       Office of Water (4305)
   &EPA
FACT  SHEET
DRAFT  INLAND TESTING MANUAL (ITM)
INTRODUCTION

The draft Inland Testing Manual (ITM) contains up-to-
date procedures to implement requirements in the Clean
Water  Act (CWA  Section 404(b)(l) Guidelines)  for
evaluation  of  potential   contaminant-related  impacts
associated with the discharge of dredged material in fresh,
estuarine, and saline (near-coastal) waters. Formally titled
"Evaluation of Dredged Material Proposed for Discharge
in Waters of the U.S.-Testing  Manual (Draft)", it was
prepared  by  a   joint   Environmental    Protection
Agency/Corps of Engineers (EPA/CE) Workgroup.  In
1991, EPA and the CE revised an Ocean Testing Manual
("Evaluation of Dredged Material Proposed for "Ocean"
Disposal - Testing Manual") for evaluation of potential
contaminant-related impacts associated with the discharge
of dredged  material in the ocean,  under  the Marine
Protection Research and Sanctuaries Act (MPRSA).

                The ITM Addresses:

•  contaminant-related impacts associated with discharges
   of dredged material in open  water disposal areas

•  contaminant-related  impacts to surface  water and
   surrounding environs associated with dredged material
   effluent discharged from confined disposal areas.

             The ITM  Does Not Address:

•  impacts associated with the dredging activity itself

•  impacts associated with the  discharge of fill material
   (except where dredged material is used for fill and
   there is a reason to believe  that contaminants may be
   released).

•  impacts  associated  with the discharge of  dredged
   material in the ocean, under the MPRSA.

BACKGROUND

Sediments   may   contain  contaminants   which,   if
bioavailable, can cause adverse environmental effects and,
in some cases, affect  human health.   Dredged material
disposal activities  may  release  or  redistribute these
contaminants. The  vast majority of disposal activities
occur in inland and near  coastal waters. The ITM  sets
                                   forth national technical guidance (which replaces a 1976
                                   guidance manual) for evaluating potential contaminant-
                                   related impacts from dredged material discharges in such
                                   waters.
                                                     THE ITM

                                              is a new document

                                              contains up-to-date procedures

                                              provides a national framework

                                              allows for regional flexibility
                                   SCHEDULE

                                   The ITM was released in early 1993 for peer review by
                                   the EPA Science Advisory Board (SAB), and for broad
                                   agency  review  and  comment.  It is being released  for
                                   public review  and comment at this time, and will be
                                   finalized in late 1994. As per the Federal Register notice
                                   announcing the availability of the ITM, a copy may be
                                   obtained from  Shirley Walker at  the  CE Waterways
                                   Experiment Station (601-634-2571).

                                   PURPOSE

                                   The  ITM provides a national testing framework which
                                   comprises one  element  of an overall decision-making
                                   process for determining whether dredged  material can be
                                   discharged into CWA Section 404  waters. The  ITM is
                                   intended to provide for consistency between  dredged
                                   material evaluations  under CWA and  MPRSA.  In
                                   recognition  of the  importance of site- and  situation-
                                   specific concerns, regional  flexibility in  implementation
                                   and application is allowed within this national framework.

                                   DESCRIPTION

                                   The ITM uses a tiered  testing approach  as shown in
                                   Figure  1 and described below.

-------
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\ EVALUATE EXISTING
1 INFORMATION; TESTING
ONLY FOR EXCLUSONS
WATER COLUMN BENTHOS
MEASURE AND
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MEASURE TOXICrrY;
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1
1 CASE-SPBCIHC
TOMCITY TESTS





CALCULATE
THEORETICAL
BIOACCUMULAT10N
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TO REFERENCE

MEASURE TOXldTY;
MEASURE
BIOACCUMULATION;
COMPARE TO FDA
UMITSANDTO
REFERENCE

CASE-SPECIFIC
TOXiaTY.
BIOACCUMULATION;
OTHER TESTS
1
TIER I
(GENERALLY REPRESENTS
EXISTING INFORMATION)
TIER U
(SOLELY CONCERNED WITH
CHEMISTRY)
TIER Ul
(GENERIC B10ASSAY
rroxiaTY AND
BIOACCUMULATiON] TESTS)
TIERIV
(SPECIFIC BJOASSAY
rroxiaTY AND
BIOACCUMULATION] AND
OTHER TESTS)
Figure 1 Overview of ITM Tiered Testing Approach
Tier 1 - Involves an examination of existing information
to determine  (1)  whether or  not there is "reason to
believe" that the dredged material needs to  be tested for
potential adverse effects;,  and  (2)  identification of any
contaminants of concern relative to testing in later tiers.
Material may be excluded from further testing if there is
reasonable assurance  that (1)  it  is  not  a  carrier of
contaminants,  or (2) it is adjacent and similar  to  the
disposal site material, and dispersal of the discharge can
be controlled.   Some limited testing may be necessary to
confirm such exclusions.

Tier II -  Is concerned solely  with sediment and water
chemistry. Tier II  provides useful information through
screening  tools, but not all possible determinations can be
reached at this tier. It presently consists of (1) measuring
dissolved  contaminants, (2)  evaluation of state  Water
Quality Standard (WQS) compliance using a numerical
mixing model, and (3)  an  evaluation of  theoretical
bioaccumulation potential for nonpolar organic chemicals.

Tier  III  -  Employs well-defined,  nationally  accepted
bioassays  including: (1) water column laboratory toxicity
tests, (2)  whole  sediment  laboratory  toxicity  tests,  (3)
whole sediment bioaccumulation tests.  Appropriately
sensitive   organisms   are   recommended,   including
benchmark  species for evaluating  the sensitivity of
regional species. Summaries of test conditions and test
acceptability criteria for all recommended bioassay species
are  also  provided. Toxicity  testing  emphasizes  acute
responses, generally survival.   Water  column  toxicity
evaluations consider mixing of the dredged material at the
discharge  site. Benthic bioaccumulation testing  provides
for the determination  of bioavailability through 28-day
exposure  tests. Tier  III  testing will usually provide
sufficient  information  for  use  in  the  overall decision-
making process for compliance with the Guidelines.
Tier IV - Will only be used in certain cases, where results
from tests in earlier tiers are insufficient to determine the
potential adverse effects of the material to be discharged.
Tier IV, like Tier III,  uses toxicity and bioaccumulation
tests, however: (1) toxicity tests may involve field (rather
than laboratory)  exposures,  different end-points (e.g.,
chronic rather  than acute), different species, or longer
laboratory  exposures;   (2)  bioaccumulation  tests  may
involve field  (rather  than laboratory)  exposures using
transplanted or resident organisms, or longer laboratory
exposures.  Tier IV can also include benthos studies.

Reference  Sediment - Is  the key to the evaluation of
dredged  material  - results  of  tests  using  reference
sediment provide  the  point of  comparison (reference
point) to which effects of dredged material are compared.
Reference  sediment  is generally  collected  outside  the
influence of previous disposal operations at the disposal
site, but near enough to the disposal site that the reference
sediment is subject to  all the same influences  (except
previously disposed dredged material) as the disposal site.
It should not be located in the immediate vicinity of spills,
outfalls, or other significant sources of contaminants, and
should have a grain size as similar as practicable to that
of the  dredged material and  disposal site sediment. The
reference  sediment concept  is the  subject of a CWA
rulemaking that will be proposed in the Federal Register
for public comment prior to issuance of the  final  ITM.
           THE ITM INCLUDES:

           Statutory and Regulatory Background
           Scope and Applicability
           Overview of Testing and Evaluation
           Technical Guidance
           -  Sampling and Analysis
           -  Physical and Chemical Evaluations
           -  Bioassays (Toxicity and
                 Bioaccumulation)
           -  Quality Assurance/Quality Control
              Evaluation of Discharges from
                 Confined Disposal Facilities
           -  Evaluation of Mixing
           -  Statistical Methods
           -  Identification of Ammonia Toxicity
SUMMARY

The  ITM  is  intended  to  provide  greater  national
consistency in  the  (1) testing process, and (2) level of
environmental protection, both among regions of the U.S.
and between inland and ocean waters.

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