The effects of various alkanes on the induction of BMO activity in lactate-grown P. butanovora. The y axis values represent BMO-specific activity as measured by the ethene oxide (EtO) assay (ethene oxide · min−1 · mg protein−1). Results of the independently conducted experiments are as follows. (A) Time course of butane (□)- and propane (○)-dependent induction of BMO activity from lactate-grown P. butanovora. (B) Induction of BMO activity following 2 h of incubation with either 224 μM butane (B1), 112 μM butane (B2), 328 μM propane (P), or a mixture of 112 μM butane and 164 μM propane (B+P). (C) Time course of the effect of ethane, propane, and pentane on butane-dependent induction. At time zero, vials containing lactate-grown cells received either 1 mmol butane (white symbols) or no n-alkane (•). At the time indicated by the arrow, vials received no additional n-alkane (Δ) or 2 mmol of either ethane (×), propane (□), butane (○), or pentane (⋄).