CombiNAber

A tool for Prediction & Analysis of Neutralization by Antibody Combinations

Purpose: This tool predicts and analyzes combination antibody neutralization scores using IC₅₀ and/or IC₈₀ for individual antibodies. The predicted scores are systematically compared for all single antibodies and 2, 3 and 4 antibody combinations analyzed. See explanation.

IC₅₀/IC₈₀ data

Paste values or upload file (See assay requirements) [Sample Input]	'<' and '>' signs are NOT allowed. Please replace them with 'LT' and 'GT' respectively.
Data type	IC50   IC80   Both
Delimiter	Comma   Space   Tab

mAb class

Paste values or upload file (See Ab class requirements)
Delimiter	Comma   Space   Tab

Options

Prediction method	Additive Bliss-Hill
mAb combinations	Combinations using full set of mAbs       # of Abs in Ab combination 2  3  4  (may be adjusted depending # of Abs)      Repeat mAbs from same class in combinations Combinations of interest (example)
Analyses	Target concentration ug/ml (seperate with commas if more than one concentration)
	Active coverage by multiple mAbs in combination 2  3  4
	Incomplete neutralization
	Instantaneous inhibitory potential (IIP)
File format for figures	PDF  SVG  PNG
Email results

Reference:

• Kshitij Wagh, Tanmoy Bhattacharya, Carolyn Williamson, Alex Robles, Madeleine Bayne, Jetta Garrity, Michael Rist, Cecilia Rademeyer, Hyejin Yoon, Alan Lapedes, Hongmei Gao, Kelli Greene, Mark K. Louder, Rui Kong, Salim Abdool Karim, Dennis R. Burton, Dan H. Barouch, Michel C. Nussenzweig, John R. Mascola, Lynn Morris, David C. Montefiori, Bette Korber and Michael S. Seaman. Optimal Combinations of Broadly Neutralizing Antibodies for Prevention and Treatment of HIV-1 Clade C Infection. PLoS Pathog. 2016 Mar 30;12(3):e1005520. PubMed.