The final Endocrine Disruptor Screening Program Test Guidelines are generally intended to meet testing requirements under TSCA, FIFRA and FFDCA to determine if a chemical substance may pose a risk to human health or the environment due to the disruption of the endocrine system.

Group A - EDSP Tier 1 Test Guidelines

890.1100 – Amphibian Metamorphosis (Frog)
890.1150 – Androgen Receptor Binding (Rat Prostate)
890.1200 – Aromatase (Human Recombinant)
890.1250 – Estrogen Receptor Binding
890.1300 – Estrogen Receptor Transcriptional Activation (Human Cell Line HeLa-9903)
890.1350 – Fish Short-Term Reproduction
890.1400 – Hershberger (Rat)
890.1450 – Female Pubertal (Rat)
890.1500 – Male Pubertal (Rat)
890.1550 – Steroidogenesis (Human Cell Line – H295R)
890.1600 – Uterotrophic (Rat)

Group B - EDSP Tier 2 Test Guidelines

890.2100 – Avian Two-Generation Toxicity Test in the Japanese Quail
890.2200 – Medaka Extended One Generation Reproduction Test
890.2300 – Larval Amphibian Growth and Development Assay (LAGDA)