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Industrial Biotechnology

Changes in Cell Wall Carbohydrate Extractability Are Correlated with Reduced Recalcitrance of HCT Downregulated Alfalfa Biomass

To cite this article:
Sivakumar Pattathil, Trina Saffold, Lina Gallego-Giraldo, Malcolm O'Neill, William S. York, Richard A. Dixon, and Michael G. Hahn. Industrial Biotechnology. August 2012, 8(4): 217-221. doi:10.1089/ind.2012.0013.

Published in Volume: 8 Issue 4: August 16, 2012
Online Ahead of Print: July 26, 2012

Author information

Sivakumar Pattathil,¹ Trina Saffold,¹ Lina Gallego-Giraldo,² Malcolm O'Neill,¹ William S. York,¹ Richard A. Dixon,² and Michael G. Hahn¹

¹Complex Carbohydrate Research Center and Department of Energy BioEnergy Science Center, University of Georgia, Athens, GA

²Plant Biology Division and Department of Energy BioEnergy Science Center, Samuel Roberts Noble Foundation, Ardmore, OK

Address correspondence to:

Michael G Hahn, Ph.D.

Professor of Plant Biology

Complex Carbohydrate Research Center and Department of Energy BioEnergy Science Center

University of Georgia

315 Riverbend Road

Athens, GA 30602

Phone: (706) 542-4457

Fax: (706) 542-4412

E-mail: hahn@ccrc.uga.edu

ABSTRACT

Downregulation of the expression of enzymes involved in lignin biosynthesis in alfalfa (Medicago sativa L.) has been shown to result in reduced cell wall recalcitrance and increased saccharification. Here we describe changes in the extractability of hemicellulosic and pectic polysaccharides in cell walls of alfalfa downregulated in the expression of hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferase (HCT). Xylan and pectic polysaccharides are more readily solubilized from the cell walls of HCT downregulated transgenic lines than from empty vector control plants, suggesting alterations in lignin-polysaccharide interactions in the transgenic plants. Our studies show that reduced recalcitrance of HCT downregulated lines is also correlated with increased xylan extractability.

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