Mass Spectrometry
Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with word-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.
Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:
- Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
- Subcellular localization, turnover rates and modification states of proteins
- Top-down proteomics and broad intact-protein level measurements
- Characterization of protein-protein/metabolite interactions
- Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.
Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.
Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.
Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.
A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC webpage.
Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:
Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with word-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.
Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:
- Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
- Subcellular localization, turnover rates and modification states of proteins
- Top-down proteomics and broad intact-protein level measurements
- Characterization of protein-protein/metabolite interactions
- Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.
Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.
Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.
Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.
A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC webpage.
Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:
Unraveling Molecular Complexity of Natural Systems
Koppenaal a co-editor of just published encyclopedia
Developing a strategy to identify & validate pharmacodynamic kinase inhibitorbiomarkers
OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS
Agile BioFoundry
A Molecular Atlas of the Developing Lung
Lipton a co-PI on major soil carbon cycle study
EMSL's annual call for proposals opens in early January
Pan-OMIC characterization of the molecular determinates of uterine receptivity
Pages
Description
Proteomics Capabilities
- High resolution and mass accuracy Fourier-transform ion cyclotron resonance (FT-ICR) spectrometers, from 6 Tesla (T) to 15T and 21T in development
- Orbitrap based platforms including Elite, Velos and Exactive mass spectrometers
- Triple-quadrupole mass spectrometers for targeted quantitation
- Gas Chromatography (GC) MS instruments with extensive compound identification libraries
- Ion mobility spectrometry (IMS) coupled to time-of-flight (TOF) mass spectrometers
- Advanced custom nano-HPLC systems, augmented by Agilent, Waters and Eksigent systems
- MALDI and C60 SIMS mass spectrometry imaging (MSI) ion sources
Aerosol Capabilities
- LTQ-Orbitrap
- Field-deployable, second-generation, single-particle, laser-ablation, TOF mass spectrometer (SPLAT II)
- Proton transfer reaction mass spectrometer
- High-resolution TOF aerosol mass spectrometer
Ion-Surface Collision Study Capabilities
- 6T FT-ICR spectrometer configured for studying ion-surface interactions
- Ion deposition instrument for preparation of novel materials using ion soft-landing
- TOF secondary ion mass spectrometer (TOF-SIMS)
Instruments
Publications
Science Highlights
Instruments
Leads
Dr. Lipton is EMSL's mass spectrometry capability lead. She is also a senior staff scientist at Pacific Northwest National Laboratory.
She is nationally recognized for her research in microbial proteomics, specifically for developing and...