750 MHz NMR Rainier (Liquids)

Highlighted Research Applications

Structural biology
- Protein structure and dynamics
- Nuclei acid structure and dynamics.
Metabolomics
- Eukaryotic and prokaryotic.
- Ecometabolomics
  - Characterization of environmental samples using multi-dimensional NMR
    - Low gamma nuclei solids investigations.

Researchers may operate the system independently and can be trained to manage fully self-directed operations. Collaborative work with staff scientists is encouraged. For remote use, the user may ship sample(s) to EMSL, and a staff scientist will assist with placing the sample(s) in the magnet and tuning the probe. The user can then operate the spectrometer via a secure Internet connection. High Pressure NMR probe capability for CO₂ sequestration and controlled gas abiotic and biotic investigations.

Solid-State NMR Probes

750 Probes	Top Speed	Range of X	VT Range	MAKE
6.0-mm HX MAS	8KHz	31P to 15N	-50C to 150C	REVOLUTION NMR
5.0-mm HX MAS	12K	50MHz to 101MHz	RT only	EMSL
5.0-mm HX MAS	12K	45MHz to 15MHz	RT only	EMSL
3.2-mm HX MAS	25 kHz	31P to 17O	RT only	AGILENT

Commercial Liquid-State NMR Probes

750 Probes	Type	TUNING RANGE	VT Range
5-mm HCN, PFG	RT probe	HCN	-20°C to +80°C
5-mm HCN, PFG	RT probe	HCN	-20°C to +80°C
5-mm HX, PFG	RT probe	31P to 15N	-20°C to 80°C

Instrument ID:

1003

Availability: 24 hours a day, 7 days a week

Custodians

David Hoyt	(509) 371-6545	david.hoyt@pnnl.gov
Sarah D Burton	(509) 371-6535	sarah.burton@pnnl.gov

Publications

Global analysis of Salmonella alternative sigma factor E on protein translation.

The alternative sigma factor E (E) is critical for response to extracytoplasmic stress in Salmonella. Extensive studies have been...

N-15 NMR spectra of naturally abundant nitrogen in soil and aquatic natural organic matter samples of the International Humic

The naturally abundant nitrogen in soil and aquatic NOM samples from the International Humic Substances Society has been characterized by solid state...

1H and 13C NMR Chemical Shift Assignments and Conformational Analysis for the Two Diastereomers of the Vitamin K Epoxide

Proton and 13C NMR chemical shift assignments and 1H-1H scalar couplings for the two diastereomers of the vitamin K epoxide reductase (VKOR)...

Magnesium Silicate Dissolution Investigated by 29Si MAS, 1H-29Si CP MAS, 25Mg QCPMG, and 1H-25Mg CP QCPMG NMR.

Olivine has been the subject of frequent investigation in the earth sciences because of its simple structure and rapid dissolution kinetics. Several...

Functionalized Silicone Nanospheres: Synthesis, Transition Metal Immobilization, and Catalytic Applications.

Silicone nanospheres containing a variety of functional groups (pyridines, phosphines, thiols, amines, etc.) have been prepared by emulsion...

Base-Promoted Ammonia Borane Hydrogen-Release.

The strong non-nucleophilic base bis(dimethylamino)naphthalene (Proton Sponge, PS) has been found to promote the rate and extent of H2-release from...

Characterization of cation environments in polycrystalline forsterite by Mg-25 MAS, MQMAS, and QCPMG NMR .

Forsterite (Mg2SiO4) is a silicate mineral frequently studied in the Earth sciences as it has a simple crystal structure and fast dissolution...

A solid-state 55Mn NMR spectroscopy and DFT investigation ofmanganese pentacarbonyl compounds.

Central transition 55Mn NMR spectra of several solid manganese pentacarbonyls acquired at magnetic field strengths of 11.75, 17.63, and 21.1 T are...

Highly Selective Olefin Epoxidation with Aqueous HO Over Surfacemodified TaSBA15 Prepared via the TMP Method.

Trialkylsiloxy-modified Ta(V) centers on mesoporous silica exhibit excellent selectivity for epoxide formation (>98% after 2 h) in the oxidation...

High-field As NMR Study of Arsenic Oxysalts.

Arsenic (bio)geochemistry is of intense current interest because the speciation, mobility, and natural chemical processes involving arsenic compounds...

Mg/Al Ordering in Layered Double Hydroxides Revealed by Multinuclear NMR Spectroscopy.

The anion-exchange ability of layered double hydroxides (LDHs) has been exploited to create materials for use in catalysis, drug delivery, and...

Quick Specs

- 750 MHz 1H frequency
- VNMRS DirectDrive console
- VnmrJ 3.1 software
- Capable of solution-state and solid-state experiments

Science Highlights

Unlocking the Potential of Peptide-based Drugs

Posted: Wednesday, September 14, 2016 - 11:19

The Science Peptides are naturally occurring molecules with excellent pharmaceutical properties, but their therapeutic use has been limited by the...

Study hints at conduction secrets in bacteria nanowires

Posted: Tuesday, November 12, 2013 - 16:00

Tiny electrical wires protrude from some bacteria and contribute to rock and dirt formation. Researchers studying the protein that makes up one such...

Projects

Analysis of Post-translational Modifications of endo-Glucanases using High Accuracy Mass Spectrometry

A critical step in the lignocellulosic biofuels pipeline is the extraction of fermentable sugars from plant biomass. Extracellular fungal enzymes are...

Behavior of Ions and H2O at NOM, Clay, and Clay-NOM Interfaces with Supercritical Fluids

Our research program strives to provide new insight into the fundamental molecular-scale dynamics, energetics, and reactivity of geochemically...

Bioinspired Homogeneous and Surface-confined Catalysts for Energy Storage and Production Reactions

Three related research programs at PNNL focus on developing bioinspired electrocatalysts for energy storage, energy production, or carbon fixation...

Characterizing the Cellular Envelope and Secreted Compounds of Anaerobic Gut Fungi

This proposal seeks use of EMSL resources to characterize the cellular envelope and secreted metabolites of anaerobic gut fungi. We have previously...

Chemical Characterization of Atmospheric Aerosols and Cloud Water using High-Resolution Mass Spectrometry and Single-Particle

High-resolution tandem mass spectrometry and single particle microscopy analysis will be utilized to determine the organic molecular composition and...