David Hoyt

David Hoyt
Staff Member Title: 
Sr. Research Scientist IV
Phone: 
(509) 371-6545
Contact Email: 
david.hoyt@pnnl.gov
Overview: 

Dr. Hoyt has had over 30 years of experience with research related to NMR. His traditional research focus has been on the structure and dynamics of bio-complexes and metabolite identification via NMR, but more recently has broadened his interests to include bio-geochemistry and NMR specialty-probe development projects. Dr. Hoyt has co-authored over 40 peer-reviewed publications including articles in Nature Microbiology, Nature Structural Biology, Molecular Cell, JACS, Biochemistry, and Journal of Magnetic Resonance.  He has worked for over 10 years in management and 19 years support of the Environmental Molecular Sciences Laboratory User Facility, providing technical support and research collaboration for various user projects in high field liquids NMR with structural biology and metabolomics applications. Led an effort to site and develop ~ 12 M in new American Reinvestment and Recovery Act funded magnetic resonance systems between June 2009 - November 2011. From 2002 to present he has helped site and develop over 24 M in NMR/EPR capital equipment to EMSL. This work included enhancements to in-situ NMR bioreactor and metabolomics capabilities. Present interests now include further developing and participating in metabolomics research in both biological and bio-geologic contexts. He will directly participate in high resolution NMR experimental design, data collection and analysis.

Details: 
  • 2011 - Present - Sr. Research Scientist IV, NMR/EPR - EMSL, PNNL
  • 2002 - 2011 - Sr. Research Scientist IV, Technical Group Lead, NMR/EPR - EMSL, PNNL
  • 2001 - 2002 - Sr. Research Scientist IV, Macromolecular Structure and Dynamics -FSD, PNNL
  • 1997 - 2001 - Sr. Research Scientist III, Macromolecular Structure and Dynamics, FSD, PNNL
  • 1995 - 1997 - Postdoctoral Fellow, Macromolecular Structure and Dynamics, FSD, PNNL
  • 1991 - 1995 - Postdoctoral Fellow, Dept. of Biochemistry, University of Alberta
Education: 
  •  Ph.D., Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas (1990).
  •  M.A., Biochemistry, University of Delaware, Newark, Delaware (1987).
  •  B.S., Chemistry, Millersville University, Millersville, Pennsylvania (1983). 
Awards & Honors: 

2015 - R&D100 Award – Winner - Pressurized Magic Angle Spinning (PMAS) Technology for Nuclear Magnetic Resonance Spectroscopy – Nov. 13, 2015 - DW Hoyt, JZ Hu, JA Sears, ED Walter, HS Mehta KM Rosso, RVF Turcu http://www.rdmag.com/news/2015/11/2015-r-d-100-award-winners

Hoyt received four individual and team PNNL Divisional Outstanding Performance Awards 1999-2011 and two EMSL Director Awards for Outstanding Contributions in 2001 and 2011. And was the recipient of the Laboratory Equipment Distinguished Manager, March 2004.

Professional Affiliations and Service

Reviewer for National Science Foundation (Chemical Research Instrumentation and Facility), Reviewer for Natural Sciences and Engineering Research Council of Canada (NSERC), Reviewer for Computing in Science & Engineering journal, ACS member, AAAS member, AGU member, Metabolomics Society member.

Patents

U.S. Patent No. 9,194,920 - Magic Angle Spinning Nuclear Magnetic Resonance Apparatus and Process for High-Resolution In Situ Investigations”- issued Nov. 24, 2015 - JZ Hu, JA Sears, DW Hoyt, HS Mehta, CHF Peden.

U.S. Patent No. 8,692,548 “Devices and Process for High-Pressure Magic Angle Spinning Nuclear Magnetic Resonance” issued April 8, 2014, DW Hoyt, JA. Sears, RVF Turcu, KM Rosso, and JZ Hu

U.S. Patent No. 7,535,224 “Discrete Magic Angle Turning System, Apparatus and Process for In Situ Magnetic Resonance Spectroscopy and Imaging” issued May 19, 2009, JZ Hu, JA Sears, DW Hoyt, and RA Wind.

US Patent Pending – No. 15/225,688 “SNAP-IN BUSHINGS AND PROCESS FOR HIGH-PRESSURE AND/OR HIGH TEMPERATURE MAGIC ANGLE SPINNING NUCLEAR MAGNETIC RESONANCE” Filed August 1, 2016,  ED Walter, JA Sears, HS Mehta, DW Hoyt.

Research Interests: 
  • Nuclear Magnetic Resonance Spectroscopy
  • Metabolomics
  • NMR technology development for controlled sample environments to aid In Situ measurements
  • Supercritical Fluid - Mineral - Microbe Interactions
  • Bio-oils and Biofuels
  • Structural Basis for Protein-Protein Interactions
  • Other Spectroscopies - CD, Fluorescence
Capabilities: 
NMR and EPR
Spectroscopy and Diffraction
Instrument(s): 
750 MHz Radiological NMR Bokan (Liquids, Solids)
800 MHz NMR Denali (Liquids)

Publications

2015

  • HB Jung, KC Carroll, S Kabilan, DJ Heldebrant, DW Hoyt, L Zhong, T Varga, SA Stephens, L Adams, A Bonneville, AP Kuprat, CA Fernandez. 2015. "Stimuli-Responsive/Rheoreversible Hydraulic Fracturing Fluids as a Greener Alternative to Support Geothermal and Fossil Energy Production." Green Chemistry 17(5)2799-2812. 10.1039/c4gc01917b

2014

  • GM Bowers, DW Hoyt, SD Burton, BO Ferguson, T Varga, RJ Kirkpatrick. 2014. "In Situ 13C and 23Na Magic Angle Spinning NMR Investigation of Supercritical CO2 Incorporation in Smectite-Natural Organic Matter Composites." Journal of Physical Chemistry C 118(7)3564-3573. 10.1021/jp410535d
  • MJ Wilkins, DW Hoyt, MJ Marshall, PA Alderson, AE Plymale, LMeng Markillie, AE Tucker, ED Walter, BE Linggi, A Dohnalkova, RC Taylor. 2014. "CO2 exposure at pressure impacts metabolism and stress responses in the model sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough." Frontiers in Microbiology 5Article No. 507. 10.3389/fmicb.2014.00507

2013

  • QRS Miller, CJ Thompson, JS Loring, CF Windisch, ME Bowden, DW Hoyt, JZ Hu, BW Arey, KM Rosso, HT Schaef. 2013. "Insights into Silicate Carbonation Processes in Water-Bearing Supercritical CO2 Fluids." International Journal of Greenhouse Gas Control 15104-118. 10.1016/j.ijggc.2013.02.005
  • J Feng, JZ Hu, SD Burton, DW Hoyt. 2013. "High Resolution Magic Angle Spinning 1H-NMR Metabolic Profiling of Nanoliter Biological Tissues at High Magnetic Field ." Chinese Journal of Magnetic Resonance 30(1)1-11.
  • RVF Turcu, DW Hoyt, KM Rosso, JA Sears, JS Loring, AR Felmy, JZ Hu. 2013. "Rotor Design for High Pressure Magic Angle Spinning Nuclear Magnetic Resonance." Journal of Magnetic Resonance 22664-69. 10.1016/j.jmr.2012.08.009
  • JS Loring, HT Schaef, CJ Thompson, RVF Turcu, QR Miller, J Chen, JZ Hu, DW Hoyt, PF Martin, ES Ilton, AR Felmy, KM Rosso. 2013. "Clay hydration/dehydration in dry to water-saturated supercritical CO2: Implications for caprock integrity." Energy Procedia 375443-54448. 10.1016/j.egypro.2013.06.463

2012

  • JZ Hu, JA Sears, HS Mehta, JJ Ford, JH Kwak, K Zhu, Y Wang, J Liu, DW Hoyt, CHF Peden. 2012. "A Large Sample Volume Magic Angle Spinning Nuclear Magnetic Resonance Probe for In-Situ Investigations with Constant Flow of Reactants." Physical Chemistry Chemical Physics. PCCP 14(7)2137-2143. 10.1039/c1cp22692d
  • JS Loring, HT Schaef, RVF Turcu, CJ Thompson, QRS Miller, PF Martin, JZ Hu, DW Hoyt, O Qafoku, ES Ilton, AR Felmy, KM Rosso. 2012. "In Situ Molecular Spectroscopic Evidence for CO2 Intercalation into Montmorillonite in Supercritical Carbon Dioxide." Langmuir 28(18)7125-7128. 10.1021/la301136w

2011

  • BK Ahring, NS Baliga, JR Frederickson, S Kaplan, HB Pakrasi, JG Pounds, I shah, S Slater, G Stacey, KJ Auberry, DR Baer, DML Brown, DE Cowley, DW Hoyt, DW Koppenaal, BE Linggi, KT Mueller, G Orr, EW Robinson, RG Teller, HS Wiley, NJ Hess, L Pasa-Tolic, KM Waters, KD Rodland. 2011. "Biological Interactions and Dynamics Science Theme Advisory Panel (BID-STAP)."
  • CT Campbell, AK Datye, GA Henkelman, RF Lobo, WF Schneider, LD Spicer, WT Tysoe, JM Vohs, DR Baer, DW Hoyt, S Thevuthasan, KT Mueller, CM Wang, NM Washton, I Lyubinetsky, RG Teller, A Andersen, N Govind, K Kowalski, BC Kabius, H Wang, AA Campbell, WA Shelton, EJ Bylaska, CHF Peden, Y Wang, DL King, MA Henderson, RJ Rousseau, J Szanyi, Z Dohnalek, D Mei, BC Garrett, D Ray, JH Futrell, J Laskin, DL DuBois, LR Kuprat, C Plata. 2011. "EMSL and Institute for Integrated Catalysis (IIC) Catalysis Workshop."
  • GE Brown, A Chaka, DK Shuh, EE Roden, CJ Werth, NJ Hess, AR Felmy, KM Rosso, DR Baer, VL Bailey, ME Bowden, JW Grate, DW Hoyt, LR Kuprat, AS Lea, KT Mueller, M Oostrom, G Orr, L Pasa-Tolic, C Plata, EW Robinson, RG Teller, S Thevuthasan, H Wang, HS Wiley, MJ Wilkins. 2011. "EMSL Geochemistry, Biogeochemistry and Subsurface Science-Science Theme Advisory Panel Meeting."
  • DW Hoyt, RVF Turcu, JA Sears, KM Rosso, SD Burton, JH Kwak, AR Felmy, JZ Hu. 2011. "IN SITU MAGIC ANGLE SPINNING NMR FOR STUDYING GEOLOGICAL CO(2) SEQUESTRATION ."
  • DW Hoyt, RVF Turcu, JA Sears, KM Rosso, SD Burton, AR Felmy, JZ Hu. 2011. "High-pressure Magic Angle Spinning Nuclear Magnetic Resonance ." Journal of Magnetic Resonance 212(2)378-385. 10.1016/j.jmr.2011.07.019
  • JH Kwak, JZ Hu, RVF Turcu, KM Rosso, ES Ilton, CM Wang, JA Sears, MH Engelhard, AR Felmy, DW Hoyt. 2011. "The Role of H2O in the Carbonation of Forsterite in Supercritical CO2." International Journal of Greenhouse Gas Control 5(4)1081-1092. 10.1016/j.ijggc.2011.05.013
  • MD White, BPeter McGrail, HT Schaef, JZ Hu, DW Hoyt, AR Felmy, KM Rosso, SK Wurstner. 2011. "Multiphase Sequestration Geochemistry: Model for Mineral Carbonation." Energy Procedia 45009-5016. 10.1016/j.egypro.2011.02.472
  • ST Teklemichael, WMH Oo, MC Matthew, ED Walter, DW Hoyt. 2011. "Acceptors in ZnO nanocrystals." Applied Physics Letters 98(23)Article No. 232112. 10.1063/1.3598411

2010

  • JH Kwak, JZ Hu, DW Hoyt, JA Sears, CM Wang, KM Rosso, AR Felmy. 2010. "Metal Carbonation of Forsterite in Supercritical CO2 and H2O Using Solid State 29Si, 13C NMR Spectroscop." Journal of Physical Chemistry C 114(9)4126 - 4134. 10.1021/jp1001308

2009

  • B Chen, X Ren, T Neville, WG Jerome, DW Hoyt, DL Sparks, G Ren, J Wang. 2009. "Apolipoprotein AI tertiary structures determine stability and phospholipid-binding activity of discoidal high-density lipoprotein particles of different sizes." Protein Science 18(5)921-935. 10.1002/pro.101
  • JZ Hu, JA Sears, JH Kwak, DW Hoyt, Y Wang, CHF Peden. 2009. "An Isotropic Chemical Shift-Chemical Shift Anisotropic Correlation Experiment Using Discrete Magic Angle Turning." Journal of Magnetic Resonance 198(1)105-110.

2008

  • K Briknarova, X Zhou, AC Satterthwait, DW Hoyt, KR Ely, S Huang. 2008. "Structural Studies of the SET Domain from RIZ1 Tumor Suppressor." Biochemical and Biophysical Research Communications 366(3)807-813. 10.1016/j.bbrc.2007.12.034

2007

  • X Ren, Y Yang, T Neville, DW Hoyt, DL Sparks, J Wang. 2007. "A complete backbone spectral assignment of human apolipoprotein AI on a 38 kDa preβHDL (Lp1-AI) particle ." Biomolecular NMR Assignments 1(1)69-71.
  • Y Yang, DW Hoyt, J Wang. 2007. "A Complete NMR Spectral Assignment of the Lipid-free Mouse Apolipoprotein A-I (ApoAI) C-terminal Truncation Mutant, ApoAI(1-216)." Biomolecular NMR Assignments 1(1)109-111. 10.1007/s12104-007-9031-2
  • S Smirnov, NG Isern, ZG Jiang, DW Hoyt, CJ Mcknight. 2007. "The Isolated Sixth Gelsolin Repeat and Headpiece Domain of Villin Bundle F-Actin in the Presence of Calcium and Are Linked by a 40-Residue Unstructured Sequence ." Biochemistry 46(25)7488-7496. 10.1021/bi700110v

2006

  • PS Brzovic, AV Lissounov, D Christensen, DW Hoyt, RE Klevit. 2006. "A UbcH5/Ubiquitin Noncovalent Complex is Required for Processive BRCA1-Directed Ubiquitination." Molecular Cell 21(6)873-880.

2005

  • C Xu, A Sivashanmugam, DW Hoyt, J Wang. 2005. "A Complete Backbone Assignment of the Apolipoprotein E LDL Receptor Binding Domain [Letter to the Editor]." Journal of Biomolecular NMR 32(2)177. 10.1007/s10858-005-6729-2
  • K Briknarova, F Nasertorabi, ML Havert, E Eggleston, DW Hoyt, C Li, AJ Olson, K Vuori, KR Ely. 2005. "The Serine-rich Domain from Crk-associated Substrate (p130Cas) is a Four-helix Bundle." Journal of Biological Chemistry 280(23)21908-21914.

2004

  • DW Hoyt, SD Burton, MR Peterson, JD Myers, G Chin. 2004. "Expanding Your Laboratory by Accessing Collaboratory Resources." Analytical and Bioanalytical Chemistry 378(6)1408-1410.

2003

  • DF Lowry, DW Hoyt, FA Khazi, JR Bagu, AG Lindsey, DM Wilson. 2003. "Investigation of the Role of the Histidine-Aspartate Pair in the Human Exonuclease III-like Abasic Endonuclease, Ape1 ." Journal of Molecular Biology 329(2)311-322.
  • K Briknarova, M Akermann, DW Hoyt, E Ruoslahti, KR Ely. 2003. "Anastellin, an FN3 Fragment with Fibronectin Polymerization Activity, Resembles Amyloid Fibril Precursors." Journal of Molecular Biology 332(1)205-215.
  • M Kreishman-Detrick, C Egile, DW Hoyt, JJ Ford, R Li, MK Rosen. 2003. "NMR analysis of methyl groups at 100-500 kDa: model systems and Arp2/3 complex.." Biochemistry 42(28)8579-8586.
  • DF Lowry, DW Hoyt, FA Khazi, JR Bagu, AG Lindsey, DM Wilson Iii. 2003. "Investigation of the role of the histidine-aspartate pair in the human exonuclease III-like abasic endonuclease, Ape1 ." Journal of Molecular Biology 329(2)311-322.

2002

  • G Chin, JD Myers, DW Hoyt. 2002. "Social Networks in the Virtual Science Laboratory." Communications of the ACM 45(8)87-92.
  • K Briknarova, S Takayama, S Homma, K Baker, E Cabezas, DW Hoyt, Z Li, AC Satterthwait, KR Ely. 2002. "BAG4/SODD Protein Contains a Short BAG Domain." Journal of Biological Chemistry 277(34)31172-31178.

2001

  • K Briknarova, S Takayama, L Brive, ML Havert, DA Knee, J Velasco, S Homma, E Cabezas, J Stuart, DW Hoyt, AC Satterthwait, M Llinas, JC Reed , KR Ely. 2001. "Structural Analysis of BAG1 Cochaperone and Its Interactions With Hsc70 Heat Shock Protein." Nature Structural Biology 8(4)349-352.
  • PL Bryant, C Harwell, AA Mrse, EF Emery, Z Gan, T Caldwell, AP Reyes, P Kuhns, DW Hoyt, LS Simeral, RW Hall, LG Butler. 2001. "Structural Characterization of MAO and Related Aluminum Complexes. 1. Solid-State 27 Al NMR with Comparison to EFG Tensors from ab Initio Molecular Orbital Calculations." Journal of the American Chemical Society 12312009-12017.
  • PS Brzovic, P Rajagopal, DW Hoyt, MC King, RE Klevit. 2001. "Structure of a BRCA1/BARD1 Complex: a Heterodimeric RING-RING Interaction." Nature Structural Biology 8(10)833-837.

2000

  • K Liu, HS Cho, DW Hoyt, TN Nguyen, P Olds, JW Kelly, D Wemmer. 2000. "Deuterium-proton Exchange on the Native Wild-type Transthyretin Tetramer Identifies the Stable Core of the Individual Subunits and Indicates Mobility at the Subunit Interface." Journal of Molecular Biology 303(4)555-565.