The interactions between a host and a pathogen are complex, representing a molecular interplay that results in either health or disease as an outcome. Understanding the nuances of these interactions on a molecular level is critical to preventing and/or treating infection. The objective of this project is to analyze the molecular components involved in host-viral interactions, in order to model the dynamics of viral infection. The potential impact of this investigation is wide reaching, including application in fundamental biology through the increased understanding of viral infection using unique viral systems, as well as application in identifying cellular targets for the development of therapeutics that would interfere with critical infection processes. Utilization of EMSL resources, specifically the Q-Exactive HF instrument purchased under this project, will be essential for generation of high quality data for use in downstream applications.