Abstract:
Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.
Citation:
Sontag RL, C Mihai, G Orr, A Savchenko, T Skarina, H Cui, JR Cort, JN Adkins, and RN Brown.2015."Electroporation of Functional Bacterial Effectors into Mammalian Cells."Journal of Visualized Experiments 95:Article No. e52296. doi:10.3791/52296
Volume:
Issue:
Pages:
Publication year:
2015