Mass Spectrometry
Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with word-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.
Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:
- Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
- Subcellular localization, turnover rates and modification states of proteins
- Top-down proteomics and broad intact-protein level measurements
- Characterization of protein-protein/metabolite interactions
- Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.
Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.
Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.
Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.
A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC webpage.
Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:
Science Theme:
Systems biology and complex mixture studies in biofuels, microbial communities, climate and environmental remediation can be analyzed with word-class separations and mass spectrometry capabilities. See a complete list of Mass Spectrometry instruments.
Resources and Techniques
Panomics - Advanced global proteomics, metabolomics, glycomics and activity-based omics research using cutting-edge tools, including customized hardware and sophisticated bioinformatics tools. This research includes:
- Confident protein identification and quantitation using stable-isotope labeling and label-free strategies
- Subcellular localization, turnover rates and modification states of proteins
- Top-down proteomics and broad intact-protein level measurements
- Characterization of protein-protein/metabolite interactions
- Activity based proteomics and other targeted proteomics strategies such as phosphoproteomics and biomarker validation.
Natural Organic Matter - Several workflows targeting different classes of organic compounds in soil and the environment have been developed.
Aerosol Particle Characterization - Real-time data is captured on environmentally relevant aerosols with high specificity and resolution using field-deployable equipment.
Ion-surface Collision - Fundamental aspects of activation, dissociation and deposition (soft-landing) of complex molecular ions are studied following collision with specially prepared surfaces using uniquely configured instrumentation.
A new state-of-the-art 21T FTICR mass spectrometer will be online this summer. To learn more about this system, the science it will advance, and its historical development, visit the 21T HRMAC webpage.
Other research resources found in EMSL and managed by Pacific Northwest National Laboratory include:
Unraveling Molecular Complexity of Natural Systems
Koppenaal a co-editor of just published encyclopedia
Developing a strategy to identify & validate pharmacodynamic kinase inhibitorbiomarkers
OHSU-PNNL NORTHWEST CO-LABORATORY FOR INTEGRATED OMICS
Agile BioFoundry
A Molecular Atlas of the Developing Lung
Lipton a co-PI on major soil carbon cycle study
EMSL's annual call for proposals opens in early January
Pan-OMIC characterization of the molecular determinates of uterine receptivity
Pages
Description
Proteomics Capabilities
- High resolution and mass accuracy Fourier-transform ion cyclotron resonance (FT-ICR) spectrometers, from 6 Tesla (T) to 15T and 21T in development
- Orbitrap based platforms including Elite, Velos and Exactive mass spectrometers
- Triple-quadrupole mass spectrometers for targeted quantitation
- Gas Chromatography (GC) MS instruments with extensive compound identification libraries
- Ion mobility spectrometry (IMS) coupled to time-of-flight (TOF) mass spectrometers
- Advanced custom nano-HPLC systems, augmented by Agilent, Waters and Eksigent systems
- MALDI and C60 SIMS mass spectrometry imaging (MSI) ion sources
Aerosol Capabilities
- LTQ-Orbitrap
- Field-deployable, second-generation, single-particle, laser-ablation, TOF mass spectrometer (SPLAT II)
- Proton transfer reaction mass spectrometer
- High-resolution TOF aerosol mass spectrometer
Ion-Surface Collision Study Capabilities
- 6T FT-ICR spectrometer configured for studying ion-surface interactions
- Ion deposition instrument for preparation of novel materials using ion soft-landing
- TOF secondary ion mass spectrometer (TOF-SIMS)
Instruments
The Agilent 4500 Series inductively coupled plasma mass spectrometer (ICP-MS) is available for all research areas requiring analysis of trace metals...
Custodian(s): Tom Wietsma
Type of Instrument:
Mass Spectrometer
More details on this instrument will be available soon.
Custodian(s): Ljiljana Pasa-Tolic
The 6-Tesla High-Field Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (FT-ICR MS), is a unique instrument designed and constructed at...
Custodian(s): Julia Laskin
This instrument is housed in EMSL. More information about this instrument will be available soon.
Custodian(s): Ljiljana Pasa-Tolic, Ron Moore
EMSL’s 21 Tesla Fourier transform ion cyclotron resonance mass spectrometer, or 21T FTICR MS, is one of only two in the world with this high of a...
Custodian(s): Ljiljana Pasa-Tolic
Publications
Post-translational modifications (PTMs) play an important role in various biological processes through changing protein structure and function. Some...
Bioremediation of chlorinated ethenes via anaerobic reductive dechlorination relies upon the activity of specific microbial populations −...
Prokaryotes, due to their moderate complexity, are particularly amenable to the comprehensive identification of the protein repertoire expressed...
Herein, we employ a combination of reduction synthesis in solution, soft landing of mass-selected precursor and product ions, and in situ time-of-...
For applications of tristructural isotropic (TRISO) fuel particles in high temperature reactors, release of radioactive Ag isotope (110mAg) through...
Science Highlights
Posted: December 05, 2016
The Science
A recent study demonstrated enormous potential for scientists to explore extremely complex molecular mixtures and systems frequently...
Posted: October 31, 2016
The Science
Marine organisms such as corals and some plankton build calcium carbonate (CaCO3) shells. The elements that make up these shells play a...
Posted: October 24, 2016
The Science
A new integrated model based on geochemical and multi-omic sequence information (DNA, RNA, protein) has been developed to explain key...
Posted: October 20, 2016
The Science
Glycoside hydrolases are microbial enzymes that play a key role in nutrient acquisition through the breakdown of cellulose—a major...
Posted: October 17, 2016
The solid-liquid interface is the most common interface in electrochemical systems. Scientists at Pacific Northwest National Laboratory and EMSL...
Instruments
The interaction between plants and their environment is of key importance in promoting sustainable, healthy, and robust plant growth. Underlying the...
The microbial activities on which humanity and ecosystems depend are determined by microbial community membership and by the resources and conditions...
Enzymatic hydrolysis of (ligno)cellulose by cellulases is key for the production of second generation biofuels which represent a long-standing...
While nitrogen management is an important agronomic trait in any cropping system, it is of particular concern for future bioenergy crops that will be...
The United States is one of the top nations for prematurely born infants and has a correspondingly high dayone infant mortality rate. Expanding our...
Leads
Dr. Lipton is EMSL's mass spectrometry capability lead. She is also a senior staff scientist at Pacific Northwest National Laboratory.
She is nationally recognized for her research in microbial proteomics, specifically for developing and...