Cell Isolation and Systems Analysis

Capability Details

• Super-resolution fluorescence structured illumination microscopy (SIM) enables 3D imaging of intact hydrated cells with 120 nm lateral resolution. Resolves protein complexes and subcellular structures using any fluorescent protein or dye.

• Stochastic optical reconstruction microscopy (STORM), also known as photo-activated localization microscopy (PALM), enables imaging intact hydrated cells with 20-30 nm resolution. Reconstructs super-resolution fluorescence images of protein complexes and subcellular structures in intact hydrated cells with unprecedented resolution.

• Combined atomic force microscopy (AFM) and STORM/PALM for 3D mapping of cellular structures with 1-10 nm resolution coupled with single-molecule and super resolution fluorescence imaging to identify distinct proteins and molecular complexes in the membrane of intact cells or organelles.

• Multi-photon fluorescence microscopy system that seamlessly integrates nonlinear excitation, laser scanning confocal microscopy, fluorescence lifetime imaging (FLIM) and differential interference contrast (DIC) imaging. Enables minimally invasive and deeply penetrating laser excitation for high-resolution 3D imaging and detection of molecular interactions in single cells, cell communities or tissues.

• Time-lapse single molecule fluorescence imaging uses total internal reflection fluorescence (TIRF) techniques to track individual molecules or organelles in live cells. Enables the study of subcellular processes over time and molecular interaction dynamics using fluorescence resonance energy transfer (FRET) in live cells.

• Two SOLiD® systems together with the Ion Proton™ provide unbiased global transcriptome analyses with high accuracy and throughput using multiplexing capability for RNA sequencing (RNA-Seq) of multiple samples in parallel. Enables global gene expression analyses, novel gene or isoform identification, and regulation of gene expression studies, such as ChIP-Seq or microRNA analyses in complex microbial communities or individual organisms.

• The Influx™ flow cytometer/cell sorter has multiple laser lines and powerful detection capabilities for high throughput analysis and sorting of distinct cells or organelles using advanced multi-parameter sorting technologies based on the presence and content of distinct genes and proteins or intracellular structures. Supports detection and sorting of nanoscale particles, making it highly suited for sorting and analyzing organelles and single cells.

• High-resolution laser capture microdissection microscope equipped with a 100x magnification objective lens and multiple fluorescence lines. Enables the enrichment of distinct organelles or isolation of single cells from cell populations or tissue sections for further analyses.

• The CyTOF® mass cytometer uses Time-of-Fight mass spectrometry in single cells to quantify the expression of multiple proteins or mRNA species, each tagged with a different stable heavy metal isotope. Enables high throughput single cell analysis of the expression of multiple proteins using antibodies or multiple genes using in situ hybridization probes.

• Bioreactors for controlled growth and monitoring of diverse microbial cells in volumes ranging from 100 µl wells to multi-liter reactors. These include the Bioscreen-C™ for automated real-time analysis of growth rate in up to 200 independent 100 µl wells; the Micro-24 MicroReactor system for 24 independently controlled 5 ml reactors; and the BioFlo® 310 benchtop fermentor-bioreactor system for larger volumes.