Microscopy
Advancement in energy, environment and biology research relies heavily on micro-, nano- and atomic-scale chemical and structural imaging. Many microscopy instruments have high-resolution imaging capabilities including complementary chemical, structural and phase information, in-situ imaging in native environments and imaging of dynamic processes with high temporal-resolution. See a complete list of Microscopy instruments.
Resources and Techniques
- Nanoscale and sub-nanoscale imaging allows users to elucidate chemical processes and acquire structural data for a variety of samples such as nanostructures and cell-surface proteins.
- Tomography yields three-dimensional reconstruction of transmission electron microscopy images generated for biological samples as well as for soft materials and samples with 3D structural heterogeneity.
- Environmental particle analysis offers knowledge about non-volatile atmospheric particle composition and hydration properties using high-pressure scanning electron microscopy equipped with energy-dispersive x-ray analysis capability.
- Environmental mode imaging techniques enable sample preservation to eliminate extensive preparation procedures that can introduce artifacts and make possible live-cell imaging and in situ imaging in liquids or controlled gas environments with high resolution microscopy.
- Dynamic imaging capability enables real-time studies of nanosecond-scale dynamic processes with unprecedented spatial resolution, such as protein-protein interactions, with contrast at the single-molecule level.
Quiet Wing for Advanced Microscopy
Seven microscopes are housed in the Quiet Wing, a space specially designed to reduce external factors, such as vibrations and electromagnetic fields, that can impede capture of high-resolution images. Read more about the Quiet Wing and its instrumentation.
A new DTEM – Dynamic Transmission Electron Microscope – is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest National Laboratory. It will be housed in the Quiet Wing. To learn more about this system, the science it will advance and its historical development, visit the DTEM page.
Science Theme:
Advancement in energy, environment and biology research relies heavily on micro-, nano- and atomic-scale chemical and structural imaging. Many microscopy instruments have high-resolution imaging capabilities including complementary chemical, structural and phase information, in-situ imaging in native environments and imaging of dynamic processes with high temporal-resolution. See a complete list of Microscopy instruments.
Resources and Techniques
- Nanoscale and sub-nanoscale imaging allows users to elucidate chemical processes and acquire structural data for a variety of samples such as nanostructures and cell-surface proteins.
- Tomography yields three-dimensional reconstruction of transmission electron microscopy images generated for biological samples as well as for soft materials and samples with 3D structural heterogeneity.
- Environmental particle analysis offers knowledge about non-volatile atmospheric particle composition and hydration properties using high-pressure scanning electron microscopy equipped with energy-dispersive x-ray analysis capability.
- Environmental mode imaging techniques enable sample preservation to eliminate extensive preparation procedures that can introduce artifacts and make possible live-cell imaging and in situ imaging in liquids or controlled gas environments with high resolution microscopy.
- Dynamic imaging capability enables real-time studies of nanosecond-scale dynamic processes with unprecedented spatial resolution, such as protein-protein interactions, with contrast at the single-molecule level.
Quiet Wing for Advanced Microscopy
Seven microscopes are housed in the Quiet Wing, a space specially designed to reduce external factors, such as vibrations and electromagnetic fields, that can impede capture of high-resolution images. Read more about the Quiet Wing and its instrumentation.
A new DTEM – Dynamic Transmission Electron Microscope – is under development at EMSL in collaboration with scientific colleagues at Pacific Northwest National Laboratory. It will be housed in the Quiet Wing. To learn more about this system, the science it will advance and its historical development, visit the DTEM page.
Attachments:
An In-situ Investigation of a-AlOOH Dissolution under High pH Conditions
Fundamental Understanding of Nucleation Processes to Assess Solution Stability and Phase Growth and Genesis
Morphology and Protein Profile of Bacterium-Membrane-Formed Nanovesicles
Investigating interactions and aggregate structures of boehmite particles toward understanding of bulk rheology
EMSL's annual call for proposals opens in early January
Advancing Materials for Quantum Information Science: CVD Growth and Functionalization of Graphene with Ordered Arrays of
In situ TEM study of hierarchical nanowires growth via screw dislocation driven mechanism
In situ TEM study of branched nanocrystal growth mechanism
Cream of the Crop: Top-Tier Publications from 2016
Pages
Description
Capability Details
• Electron microscopes with tomography, cryo, scanning, photoemission and high-resolution (sub-nanometer) imaging capabilities
• Focused ion beam/scanning electron microscopes for specialized sample preparation and three-dimensional topographic and chemical imaging
• Nuclear magnetic resonance microscopy with 10-40-_m resolution to study the anatomy, metabolism and transport processes of live cell cultures, biofilms and tissue samples
• Dual Raman confocal microscope for analysis of radiological samples
• Single-molecule fluorescence tools to study molecular interactions in real time
• Scanning probe microscopy with capabilities ranging from examination of dynamic nanoscale processes in condensed environments to high resolution studies of catalysis materials in ultra-high vacuum.
Instruments
The LEAP® 4000 XHR local electrode atom probe tomography instrument enabled the first-ever comprehensive and accurate 3-D chemical imaging studies...
Custodian(s): Arun Devaraj, Daniel Perea
This FEI Tecnai T-12 cryo-transmission electron microscope (TEM) complements EMSL's broader microscopy suite and JEOL 2010 analytical high-...
Custodian(s): Alice Dohnalkova
The Asylum MFP-3D BIO is a versatile atomic force microscope (AFM) that combines molecular resolution imaging and picoNewton force-based...
Custodian(s): Kevin M. Rosso
Housed in EMSL's RadEMSL (Radiochemistry Annex), the field emission electron microprobe (EMP) enables chemical analysis and imaging of radionuclides...
Custodian(s): Bruce Arey
The environmental scanning electron microscope (ESEM) is a new-generation SEM that can image samples under controlled environments and temperatures...
Custodian(s): Alexander Laskin, Scott Lea
Publications
Surface functionalized magnetic nanoparticles (MNPs) are appealing candidates for analytical separation of heavy metal ions from waste water and...
Since the advent of the transmission electron microscope (TEM), continuing efforts have been made to image material under native and reaction...
Widespread use of silver nanoparticles raises questions of environmental impact and toxicity. Both silver particles and silver ions formed by...
LiF–ThF4 molten salt (MS) is the fuel for advanced MS reactors. Knowledge of the microscopic MS structure and dynamics is required for an...
Surface reactions of formaldehyde with reduced TiO2(110) surfaces have been studied using variable-temperature scanning tunneling microscopy (STM)...
Science Highlights
Posted: October 31, 2016
The Science
Marine organisms such as corals and some plankton build calcium carbonate (CaCO3) shells. The elements that make up these shells play a...
Posted: September 23, 2016
The Science
Wetland environments are effective at mitigating migration of many groundwater contaminants because of their unique combination of...
Posted: September 14, 2016
The Science
Brown rot fungi can decompose wood more quickly than white rot fungi, despite having fewer biodegrading enzymes for plant cell wall...
Posted: September 13, 2016
The Science
Sodium-ion batteries are an attractive alternative to lithium-ion batteries due to their high efficiency and low cost, but development...
Posted: July 29, 2016
The Science
Gold nanocrystals are industrial catalysts and are also widely used in diagnostic assays and therapeutics. A recent study revealed a...
Instruments
Electrical, optical and mechanical properties of inorganic nanostructures have strong relationships with their morphologies. For example, one-...
The objective of this study is to investigate the mobility of uranium after reaction of contaminated sediments with phosphate solutions injected at...
The first goal of this research program is to develop a robust method to quantify the atomic scale changes in structure, composition and bonding that...
Quantum information science is an up and coming area of research aimed to transform computing capabilities in the future. The use of quantum bits, or...
The goal of this proposal is to understand the microstructural evolution during non-equilibrium, rapid solidification of a molten magnesium-aluminum-...
Leads
(509) 371-6233
Dr. Lea provides scientific and technical expertise and leadership in support of EMSL’s user program, strategy and scientific productivity through leadership of microscopy capability and Quiet Wing for advanced microscopy. He also oversees...