Overview
Employers of safety-sensitive transportation employees play a vital role in ensuring the safety of their employees and the traveling public. Employers are responsible for developing and implementing successful DOT workplace drug and alcohol programs that have as their components clear policies, provisions for education and training, drug and alcohol testing, and when needed, referral for evaluation and treatment.
DOT regulated employers must comply with 49 CFR Part 40 and applicable DOT Agency regulations. Find out if you are covered under the DOT program by going to: Am I Covered?
If you are considered a covered employer, you should know about the regulations, guidelines, and supporting publications.
The following links and documents are provided as your “one-stop shopping” resource for DOT drug and alcohol testing information.
Agency Information
Quick Links
- General Information
- Federal Aviation Administration (FAA)
- Federal Motor Carrier Safety Administration (FMCSA)
- Federal Railroad Administration (FRA)
- Federal Transit Administration (FTA)
- Pipeline and Hazardous Materials Safety Administration (PHMSA)
- United States Coast Guard (USCG)
- Related Industry Links
General Information
- DOT "Recreational" Marijuana Notice
- DOT "Medical" Marijuana Notice
- Employer Brochure - Monitoring Collection Sites (**NEW**)
- Employer Handbook
- DOT’s Direct Observation Procedures
- DOT's 10 Steps to Collection Site Security and Integrity Video
- DOT Mock Collection Video
- Best Practices for DOT Random Drug and Alcohol Testing
- Current list of HHS certified laboratories
- Current list of HHS certified laboratories with approved Electronic Custody and Control Form (eCCF) systems
- DOT Agency/USCG drug and alcohol program manager contact information
- DOT Agency/USCG drug and alcohol program facts
- Employees Covered Under DOT Testing Regulation 49 CFR Part 40
- Drug and Alcohol Record Keeping Requirements
- Drug and Alcohol Testing Data MIS Collection Form and Instructions
- Employer Responsibilities under Subpart B of 49 CFR Part 40
- ODAPC - Fact Sheet
- Random Testing Rates
- Release of Information Form 49 CFR Part 40 – Drug and Alcohol Testing
DOT Agency / USCG Specific Information
Federal Aviation Administration (FAA)
- 14 CFR Part 120
- Video Series
- Forms
- How to Start a Drug and Alcohol Testing Program
- Inspection Guide
- Policy and Guidance
- Regulations
- Reporting Requirements
Federal Motor Carrier Safety Administration (FMCSA)
- Overview of the drug and alcohol rules
- Federal Alcohol and Drug Testing Requirements – Informational Brochure for Motor Carriers
- Implementation Guidelines for Motor Carrier Drug & Alcohol Testing Program
- FMCSA Medical Program for non-drug and alcohol testing, prescription-based inquiries
- Be a Driver in the know…Federal Drug and Alcohol Testing Regulations
- Drug and Alcohol Testing Brochure for Drivers
- Drug and Alcohol FAQs
Federal Railroad Administration (FRA)
- 49 CFR Part 219
- FRA Post Accident Testing Criteria
- FRA Post Accident Testing Forms
- FRA Program and Guidance Materials
- What You Need to Know About Federal Drug and Alcohol Testing - Information for FRA Covered Employees
Federal Transit Administration (FTA)
- 49 CFR Part 655
- FTA Drug and Alcohol Program
- Implementation Guidelines for Drug and Alcohol Regulations in Public Transportation
- Prescription and Over-the-Counter Medications Tool Kit
- Reasonable Suspicion Training for Supervisors Video
- Drug and Alcohol Compliance Program - Audit Questions
Pipeline and Hazardous Materials Safety Administration (PHMSA)
- 49 CFR Part 199
- PHMSA Drug and Alcohol Testing Program
- Drug and Alcohol Inspection Forms
- Random Drug Testing Selection Guidance
United States Coast Guard (USCG)
Industry and Association Links
- American Association of Medical Review Officers
- American College of Occupational and Environmental Medicine (ACOEM)
- American Society of Addiction Medicine
- Drug & Alcohol Testing Industry Association
- Drug-Free Workplace Programs
- Employees Assistance Professionals Association
- Institute for Drug-Free Workplace
- Medical Review Officer Certification Council
- National Council on Alcoholism & Drug Dependence, Inc.
- National Institute on Drug Abuse
- Office of National Drug Control Policy
- Partners for a Drug-Free America
- Substance Abuse and Mental Health Services Administration
- Substance Abuse Program Administrators Association