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Author(s): | Reiner, J. E.; Kasianowicz, J. J.; Nablo, B. J.; Robertson, J. W.; |
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Title: | Theory for Polymer Analysis Using Nanopore-based Single-molecule Mass Spectrometry |
Published: | June 01, 2010 |
Abstract: | Nanometer-scale pores have demonstrated potential as a tool for the electrical detection and characterization of molecules. To provide a physical basis for nanopore-based analytical metrology, we show that a simple model quantitatively describes how one type of analyte (polymers of poly(ethylene glycol), PEG) reduces the ionic current in a single α-hemolysin ion channel. Two critical observations are apparent in the data. The depth of PEG-induced current blockades and the residence time of the polymer in the nanopore both decrease with increasing applied potential. A model is presented which describes these phenomena by analyzing the polymer size, and cation interactions with the polymer under moderate confinement. |
Citation: | Proceedings of the National Academy of Sciences of the United States of America |
Volume: | 107 |
Pages: | pp. 12080 - 12085 |
Keywords: | bioelectronics; nanopore sensing; single molecule analysis |
Research Areas: | Nanobiotechnology |
PDF version: | Click here to retrieve PDF version of paper (1104 K) |