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Laboratory - Scientific Services Division

Tobacco Methods

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Analyte(s) Product Type Instrumentation References

Nicotine/Nicotine Alkaloids

All Tobacco Products

Flow Analyzer

1

Capillary Electrophoresis

2

Total Reducing Sugars (Sucrose, Fructose, and Glucose)

Cigars
Cigarettes
Roll-your-own
Pipe tobacco

Flow Analyzer

3

Ion Chromatography

4, 5

Reducing Sugars (Fructose and Glucose)

Cigars
Cigarettes
Roll-your-own
Pipe tobacco

Flow Analyzer

6

Ion Chromatography

4, 5

Sieve Test

Chewing tobacco
Snuff

Electric 8 inch sieve shaker, ROTAP (Reg. TM)

7

Product weight

Cigars
Cigarettes

Environmental chamber
and analytical balance

8
  1. Astoria-Pacific Method A262 (contact Astoria-Pacific International for more information)
  2. Clarke, M.B., (2002) Quantitation of Nicotine in Tobacco Products by Capillary Electrophoresis J. AOAC Intl, Vol 85, No. 1
  3. Astoria-Pacific Method A250 (reproduced with permission from Astoria-Pacific International).
  4. Zook, C.M., Patel, P.M., LaCourse, W.R., Ralapati, S, (1996) Characterization of Tobacco Products by High-Performance Anion Exchange Chromatography-Pulsed Amperometric Detection, J. Agric. Food Chem., Vol 44, No. 7
  5. Dionex Technical Note 20, Analysis of Carbohydrates by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (2000), www.dionex.com
  6. Astoria-Pacific Procedure A041 (contact Astoria-Pacific International for more information)
  7. ATF Procedure 87-4, Testing Methods Used to Distinguish Between Chewing Tobacco and Snuff
  8. TTB directive to industry (pending)

Tobacco Links:

CORESTA - An association founded in 1956, ruled by French law, the purpose being to promote international cooperation in scientific research relative to tobacco.