DRP000036 Comprehensive identification and characterization of the binding sites of polymerase II
Study Type:
Transcriptome Analysis
Submission:
DRA000036 by on 2009-11-26 18:59:00
Abstract:
Comprehensive identification and characterization of the binding sites of
polymerase II in mammalian genes were attempted. We used ChIP-Seq method, in which next
gene sequencing technology and chromatin-immunoprecipitation assay were
combined.
Description:
Although recent studies have revealed that the majority of human genes
are subjected to regulation of alternative promoters (APs), the biological relevance of
this phenomenon remains unclear. In order to understand biological significance of the
presence of divergent transcription initiation events in the respective cell types, it
is indispensable to obtain bird-view of the transcriptome figures at every step of the
gene expression; namely, i) how the genomic structure change to transcriptionally active
form, ii) where the transcription initiation complex is recruited, iii) to what extent
the transcription is activated, iv) what transcripts formed and sorted to what
subcellular fractions. We have recently started multi-faceted use of the Illumina GA to
answer these questions. Integrative analysis produced for respective aspects of the gene
expression regulations revealed the comprehensive figures of the complex human gene
transcriptome for the first time.
Center Project:
Integrateve Transcriptome Analysis
External Link:
DBTSS
BioProject:
34559 (primary)
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Experiments
| Show RUNs for each experimentHide RUNs for each experiment | |||
| ⇑Accession | Spots | Bases | |
|---|---|---|---|
| Total: 1 | 15.8 M | 567.5 M | |
 |
DRX000060 | 15.8 M | 567.5 M |
| DRR000135 | 15.8 M | 567.5 M | |